下调miR-181a对人胃癌细胞生物学行为的影响  

The effect of down-regulating miR-181a on biological behavior of gastric cancer cell

作　　者：余钧辉[1] 郑见宝[1] 孙学军[1] 王训凯 李孝彬 吴云桦 高琪[1] 张立[2] 

机构地区：[1]西安交通大学第一附属医院普通外科,陕西西安710061 [2]西安交通大学第二附属医院普通外科,陕西西安710004

出　　处：《现代肿瘤医学》2016年第10期1513-1517,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81101874;81172362);陕西省科技统筹创新工程计划资助项目(编号:2013KTCQ03-08)

摘　　要：目的:探讨下调miR-181a对人胃癌细胞生物学行为的影响。方法:转染miR-181a inhibitor下调miR-181a在人胃癌细胞SGC-7901中的表达。通过MTT法、流式细胞仪、Annexin-V+PI双染色法和Transwell实验,观察下调miR-181a表达对人胃癌细胞增殖、凋亡及侵袭迁移等生物学行为的影响。结果:瞬时转染miR-181a inhibitor后胃癌细胞SGC-7901中miR-181a的表达明显下调(P=0.002 7)。转染miR-181a inhibitor后7901细胞增殖明显减弱(P=0.000 6),被阻滞的G0/G1期细胞明显增多(P=0.007 5),S期细胞明显减少(P=0.002 2),细胞凋亡比例增加(P<0.001 7)。Transwell实验证实下调miR-181a表达能促进人胃癌细胞侵袭与迁移(P=0.000 6、P=0.000 4)。结论:下调miR-181a表达抑制胃癌细胞增殖、促进凋亡,促进侵袭迁移,提示miR-181在胃癌的发生发展中可能发挥重要的作用。Objective：To investigate the influence of down -regulating miR - 181a on biological behavior of gastric cancer cell. Methods： miR- 181a inhibitor was transfected transiently into gastric cancer cell SGC -7901 to down - regulate the expression of miR - 181a. The influence of down - regulation of miR - 181a on cell proliferation, apoptosis,invasion and migration were evaluated by MTT,flow cytometry,Annexin -V ＋ PI and transwell assay. Results： The transfection of miR- 181a inhibitor down- regulated miR- 181a expression in SGC -7901 （P = 0. 002 7）. In SGC -7901 cell after transfection with miR- 181a inhibitor, the proliferative ability was inhibited （P = 0. 000 6）, G0/G1 phase cell ratio was arrested （P = 0. 007 5）, S phase cell ratio was decreased （P = 0. 002 2）, and apoptosis rate was increased （P 〈 0. 001 7）. Transwell assay showed cell invasion and migration was increased greatly in miR - 181 a down - regulated SGC - 7901 cell （ P = 0. 000 6, P = 0. 000 4 ）. Conclusion ： Down - regulation of miR - 181 a inhibits gastric cancer cell proliferation and promotes apoptosis, but enhances cell invasion and migration. It may play an important role in gastric carcinogenesis.

关 键 词：miR-181a 胃癌 增殖 凋亡 侵袭 转移 

分 类 号：R735.2[医药卫生—肿瘤]