白杨素诱导白血病细胞HL-60凋亡及其机制  

Chrysin induces apoptosis in HL-60 cells and its mechanisms

作　　者：张嵘[1] 王洪涛[1] 苗苗[1] 李佳[1] 杨莹[1] 刘卓刚[1] 

机构地区：[1]中国医科大学附属盛京医院血液内科,辽宁沈阳110004

出　　处：《现代肿瘤医学》2016年第10期1522-1524,共3页Journal of Modern Oncology

摘　　要：目的:检测白杨素诱导白血病细胞HL-60凋亡及其机制。方法:分别利用MTT实验和Annexin V-FITC/PI双染实验检测细胞增殖和凋亡。Western blot技术检测相关通路蛋白变化。结果:白杨素可以明显诱导白血病细胞HL-60凋亡。白杨素处理后细胞的p-AKT蛋白水平明显下降。结论:白杨素能够通过AKT通路诱导HL-60细胞凋亡。Objective：To detect the effects and mechanisms of Chrysin on human leukemia cell HL -60. Methods：The proliferation and apoptosis of HL -60 cells after treated with Chrysin were detected by using MTT assay and Annexin V- FITC/PI double staining, respectively. Western blot was used to detect the mechanism of Chrysin. Results：Chrysin could induce the apoptosis in HL -60 cells. The level of p - AKT was lower in treated cells than untreated ones. Conclusion： Chrysin could induce apoptosis in HL- 60 cells by suppressing p- AKT.

关 键 词：HL-60细胞 白杨素 增殖 凋亡 AKT 

分 类 号：R733.7[医药卫生—肿瘤]