ZIC1联合苦参碱对人乳腺癌MDA-MB-231细胞增殖、迁移及凋亡的影响  

Effect of ZIC1 associated with matrine on proliferation,migration and apoptosis in MDA-MB-231 human breast cancer cell line

作　　者：于民[1] 曹方[2] 朱荣[2] 丁厚中[2] 

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]江苏大学附属昆山医院,江苏昆山215300

出　　处：《现代肿瘤医学》2016年第10期1528-1533,共6页Journal of Modern Oncology

摘　　要：目的:探讨ZIC1联合苦参碱对人乳腺癌MDA-MB-231细胞的增殖、迁移能力及凋亡的影响。方法:将慢病毒载体r LV-Zic1-PGK-puro稳定转染人乳腺癌MDA-MB-231细胞株并得到稳定传代的细胞株,Western blot法检测转染效果。以人乳腺癌MDA-MB-231细胞及ZIC1基因稳定转染的细胞为研究对象进行药物实验,MTT法筛选出苦参碱的半数抑制浓度(IC50)后分组,划分A空载不加药组(阴性对照组),B空载加药组,C转染不加药组和D转染加药组。对四组细胞采用黏附试验检测细胞黏附(增殖)能力、划痕实验检测细胞迁移能力、流式细胞术检测细胞凋亡。结果:ZIC1或苦参碱单独作用均可抑制乳腺癌MDA-MB-231细胞黏附(增殖)、迁移及增强其凋亡,差异较对照组有统计学意义(P<0.05),且在抑制细胞迁移上存在时间依赖性。两者联合作用抗癌效果更明显,差异较其它三组有统计学意义(P<0.05)。结论:苦参碱联合ZIC1可明显增强对人乳腺癌MDA-MB-231细胞的抑癌效果。Objective ：To investigate the effect of ZIC1 associated with matrine on proliferation, migration and apoptosis in MDA- MB -231 human breast cancer cell. Methods ：ZIC1 gene was transfected into human breast cancer MDA- MB- 231 cell line stably by lentiviral vector rLV- Zicl -PGK- puro and get a stable transfected cell line, then western blot analysis was used to detect expression of ZIC1. The human breast cancer MDA -MB -231 cell and the stable transfected cell was taken as the research object, use MTT assay to screen out half maximal inhibitory concentration （ ICs0 ） of matrine and conduct a grouping experiment. The cells were divided into four groups ： no - load / no - dosing group （ A）, no - load/dosing groups （ B ）, no - dosing/transfection group （ C ） and dosing/transfection group （D）. Cell viability was detected by cell adhesion test, cell migration was analyzed by wound - healing assay, the apoptosis rates were measured by flow cytometry. Results： Both ZIC1 and matrine can decrease cell adhesion （growth） , migration of MDA - MB - 231 breast cancer cell and induce its apoptosis, there was statistical significance （P 〈 0.05 ） , and the inhibitory effect on migration increased in a time - dependent manner, while the anti - tumor effect of combination of them was more apparent than the other three groups, showing statistical significance （P 〈 0.05 ）. Conclusion ：ZIC1 associated with matrinecan significantly enhance the effect of tumorinhibition on MDA- MB -231 human breast cancer cell.

关 键 词：ZIC1 苦参碱 MDA-MB-231 增殖 迁移 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]