滚环扩增技术最新研究动态及展望  

Progress and Prospects of Rolling Circle Amplification

作　　者：孙亚军[1] 王亮[1] 蔡俊[1] 

机构地区：[1]湖北工业大学工程技术学院,发酵工程教育部重点实验室,武汉430068

出　　处：《生物技术进展》2016年第2期130-136,共7页Current Biotechnology

摘　　要：滚环扩增技术(rolling circle amplification,RCA)的建立模拟了自然界中环状病原生物DNA通过滚环模型方式自我复制的原理,经长期科学研究和实践应用,取得了诸多突破性成果。对最近几年在滚环扩增技术研究领域的最新动态进行了较全面的总结,其中包括了网状RCA、锁式探针RCA、目标成环RCA和跨越式RCA,也对滚环扩增中存在的问题进行了探讨,重点介绍了该技术在基础研究、实际检测、医疗诊断及纳米材料等方面的应用,最后对核酸等温扩增技术产业化的发展前景进行了展望。The establishment of rolling circle amplification simulates the principle of self-replication of the ring-shaped pathogen DNA through the rolling circle model in nature,experiencing long-term scientific research and practical application,many breakthroughs have been achieved. This paper made a comprehensive summary on the latest research in recent years on rolling circle amplification（ RCA）,including net RCA,padlock probe RCA,target circle RCA and stride RCA. The paper also discussed the problems in the process of rolling circle amplification. We mainly introduced the application of RCA in basic research,the practical detection,medical diagnosis and Nano-materials. At last,the expectation towards the development of isothermal amplification of nucleic acid was prospected.

关 键 词：RCA 纳米材料 转基因 锁式探针 

分 类 号：Q78[生物学—分子生物学]