甲胎蛋白和α-L-岩藻糖苷酶及乙肝病毒核酸水平与原发性肝癌的关系  

The Relationship Between Hepatic Carcinoma and the Serum Levels of Alpha-fetoprotein,Alpha-L-fucosidase and Hepatitis B DNA

作　　者：刘英丽[1] 张青云[1] 

机构地区：[1]北京大学肿瘤医院暨北京市肿瘤防治研究所检验科,北京100044

出　　处：《标记免疫分析与临床》2016年第3期241-243,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨乙肝病毒核酸(HBV-DNA)水平、血清甲胎蛋白(AFP)及α-L-岩藻糖苷酶(AFU)与原发性肝癌(PHC)的关系以及三者之间的联系。方法收集北京大学肿瘤医院2014年1月至2014年12月入院患者187例(乙型肝炎组71例,肝硬化组16例,原发性肝癌组100例),健康体检者100名。患者分组参照乙型肝炎和原发性肝癌的诊断标准进行。对收集的标本采用实时荧光定量方法检测HBV-DNA水平,电化学发光法检测AFP浓度,湿化学速率法检测AFU浓度,然后对结果进行统计分析。结果肝癌组AFP和AFU水平显著高于其他三组(P<0.05),差异有统计学意义;以病毒复制情况分为无拷贝、低拷贝和高拷贝,各组间拷贝水平差异有统计学意义(P<0.05),其中肝癌组以低拷贝为主(45%);肝癌组AFP和AFU在病毒无拷贝、低拷贝和高拷贝三组间水平差异无统计学意义(P>0.05)。结论肝癌的发生是肝细胞逐渐受损伤并最终发生癌变的一个过程,乙肝病毒是其中一个主要的诱发和促进因素,因此应定时检测HBVDNA的水平,判断病毒的复制情况,并同时检测AFP和AFU的浓度,以期尽早发现肝组织的损害程度,阻止或延缓病情的发展。Objective To explore the diagnosis value of the serum levels of alpha-fetoprotein（ AFP）,alpha-Lfucosidase（ AFU） and hepatitis B DNA for Hepatic carcinoma. Methods 187 patients（ 71 patients with benign liver,16 patients with Cirrhosis liver,and 100 patients with hepatic carcinoma） and 100 cases healthy controls were involved in this study. The serum levels of HBV-DNA,AFP and AFU were detected by using real-time fluorescence quantitative method,chemiluminescence detection and the rate method of chemical respectively,and then the results were statistically analyzed. Results The level of AFP and AFU in HCC group was significantly higher than the other three groups（ P〈0. 05）. The team was divided into no copy,low copy and high copy by Viral replication,and the copy levels between the groups were statistically different（ P〈0. 05）.The level of AFP and AFU had no significant difference in the HCC patients between the virus-free copy,low copy and high copy（ P〉0. 05）. Conclusion Liver cancer is a gradual process of hepatocellular damage and eventually occurred carcinogenesis. Hepatitis B virus is one of the main factors that induce and promote PHC.Therefore patients should regularly tested the level of HBV-DNA,AFU and AFP concentration at the same time,in order to master the condition of liver tissue,and to prevent or delay the development of the disease.

关 键 词：HBV-DNA AFP AFU 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]