高强度聚焦超声联合液态氟碳纳米颗粒消融治疗宫颈癌移植瘤的实验研究  

Experimental study on anti-tumor effect of high intensity focused ultrasound ablation combined with liquidfluorocarbon nanoparticle on cervical cancer

作　　者：武佳薇[1] 张新玲[1] 尹庭辉[1] 郑荣琴[1] 肖汀[1] 毛永江[1] 

机构地区：[1]中山大学附属第三医院超声科,广州510630

出　　处：《中华超声影像学杂志》2016年第3期248-251,共4页Chinese Journal of Ultrasonography

基　　金：基金项目：国家自然科学基金青年科学基金项目（81201178）;广东省科技计划项目（2014A020212486）

摘　　要：目的探讨高强度聚焦超声（highintensityfocusedultrasound，HIFU）联合自制的包裹液态氟碳的脂质纳米颗粒治疗裸鼠宫颈癌SiHa细胞移植瘤的疗效。方法本研究按照细胞实验和动物实验两部分进行。细胞实验分为3组：对照组、HIFU组和HIFU＋PFB纳米颗粒组，CCK-8测定人宫颈癌SiHa细胞活性。动物实验亦分为3组：生理盐水组、HIFU＋生理盐水组和HIFU＋PFB纳米颗粒组，每组3只，处理后取肿瘤组织进行氯化硝基四氮唑蓝（TTC）染色，测量坏死组织面积比，病理切片行HE染色。结果细胞实验结果：HIFU辐照后，H1FU＋PFB纳米颗粒组的细胞存活率[（40．5±9．7）％]低于HIFU组[（77．7±8．5）％]及对照组[（100±4．8）]％，差异均具有统计学意义（P〈0．05）。动物实验TTC染色结果显示，同样HIFU强度辐照，HIFU＋PFB纳米颗粒组肿瘤组织发生明显大范围坏死，坏死面积比为（65．97±25．10）％，HIFU＋生理盐水组坏死面积比为（34．14±12．20）％，生理盐水组无明显坏死组织，各组间差异具有统计学意义（P〈0．05）。HE染色可见：HIFU十PFB纳米颗粒组大部分组织坏死，肿瘤细胞结构消失，周边可见核固缩核碎裂，HIFU组坏死范围则较小。结论HIFU联合包裹液态氟碳的脂质纳米颗粒可有效消融宫颈癌移植瘤。Objective To investigate the therapeutic effect of high intensity focused ultrasound （HIFU） ablation combined with homemade liquid fluorocarbon nanoparticles on cervical cancer in nude mice. Methods The cell experiment was divided into three groups： a, control group; b, HIFU group;c, HIFU ＋ PFB nanoparticle group, and the viability of cells was detected using CCK-8 reagent. The mice were also divided into three groups ： A, 0.9 % NaCl group ; B, HIFU ＋ 0.9 % NaCl group ; C, HIFU ＋ PFB nanoparticle group. The tumors were removed and underwent triphenyl tetrazolium chloride （TTC） staining, and the necrosis area was measured. Histopathological changes of the tumors were examined by light microscopy. Results After HIFU irradiation, the viability rate of group c was （40.5 ± 9.7）%, it was lower than that of group b （77.7±8.5）% （P 〈0.05） and that of group a（100±4.8）% （P 〈0.05）. TTC staining of tumor showed a large scale of necrotic tissue in group C. The necrosis ratio of the three groups was0%,（34.14±12.2）% and （65.97±25.1）%,respectively （P 〈0.05）. HE staining showed karyorrhexis or an absence of nuclei in group B and group C, which demonstrated the coagulation necrosis. Conclusions HIFU ablation combined with liquid fluorocarbon nanoparticles can effectively treat the xenograft model of the human cervical carcinoma in nude mice.

关 键 词：高强聚焦超声 纳米粒子 宫颈肿瘤 液态氟碳 

分 类 号：R737.33[医药卫生—肿瘤] R445.1[医药卫生—临床医学]