阿司匹林对兔颊VX-2鳞状细胞癌炎症微环境中树突细胞的影响  

Effects of aspirin on dendritic cells in the inflammatory microenvironment of rabbit buccal VX-2 squamous cell carcinoma

作　　者：陈志红[1] 黄桂林[2] 张霓霓[2] 易杰[2] 姚礼[2] 张林[2] 

机构地区：[1]贵州省人民医院口腔科,贵阳550002 [2]遵义医学院附属口腔医院口腔颌面外科,遵义563000

出　　处：《华西口腔医学杂志》2016年第2期178-182,共5页West China Journal of Stomatology

基　　金：遵义市科技局科研基金[遵市科合社字(2013)32号]~~

摘　　要：目的探索阿司匹林及炎症对兔颊VX-2鳞状细胞癌上清液中树突细胞(DC)成熟及功能的影响。方法通过瘤粒植入术及机械创伤+高糖饮食的方法,建立兔颊VX-2鳞状细胞癌及其炎症模型,将模型兔分为3组。实验组:制造颊癌及炎症后使用阿司匹林灌胃治疗3 d;对照组:制造颊癌及炎症后以生理盐水灌胃作为对照;空白组:不制造炎症的单纯肿瘤兔组。3 d后收集各组肿瘤标本,制成匀浆,取上清液与正常兔外周血单核细胞共培养以使其向DC分化,采用流式细胞仪检测DC表面分子CD83、CD86、人类白细胞DR抗原(HLA-DR)的表达,混合淋巴细胞反应检测DC刺激T细胞增殖的能力。结果实验组和对照组中CD83、CD86、HLA-DR阳性率均低于空白组(P<0.05),而实验组和对照组间的差异无统计学意义(P>0.05);同样,实验组和对照组刺激T细胞增殖的能力弱于空白组(P<0.05),而两组间刺激T细胞增殖的能力无明显差异(P>0.05)。结论炎症可抑制DC表面分子CD83、CD86、HLA-DR的表达和功能发挥,短期、小剂量服用阿司匹林对兔颊VX-2鳞状细胞癌炎症微环境中DC的表型改善和功能发挥无明显作用。Objective To explore the effects of aspirin and inflammation on the maturation and function of dendritic cells（DC） on the supernatant of VX-2 squamous cell carcinoma. Methods The rabbit buccal VX-2 squamous cell carcinoma models with inflammation were established by tumor particle implantation, mechanical trauma, and high sugar diet. The rabbits were divided into three groups. For the experimental group（rabbit buccal VX-2 squamous cell carcinoma with local inflammation）, aspirin were given by gavage for three consecutive days. For the control group（rabbit buccal VX-2 squamous cell carcinoma with local inflammation）, normal saline was given by gavage for three consecutive days. For the blank group（tumor without inflammation）, normal saline was given by gavage for three consecutive days. Each tumor specimens were collected in three days and made into tissue homogenate. The supernatant was collected after centrifugation. Normal rabbit peripheral blood mononuclear cells were separated and co-cultured with different states of supernatant. The expression of DC surface markers CD83, CD86, and human leukocyte antigen-DR（HLA-DR） were detected by flow cytometry. The state of function of DC was tested by mixed lymphocyte reaction. Results The positive rate of CD83, CD86, and HLA-DR of the experimental and control groups were both lower than that of the blank group（P〈0.05）. In addition, the ability to stimulate T cell proliferation of the experimental and control groups were weaker than that of the blank group（P〈0.05）. No significant difference was observed between the experimental and control groups（P〈0.05）. Conclusion Inflammation may inhibit the function and expression of CD83, CD86, and HLADR of DC. The short-term administration of aspirin is not conducive to the phenoty and function of DC in a rabbit buccal VX-2 squamous cell carcinoma inflammatory environment.

关 键 词：阿司匹林 炎症 VX-2鳞状细胞癌 树突细胞 

分 类 号：R739.8[医药卫生—肿瘤]