Id-1在结直肠癌中的表达及其临床意义  

Expressions of Id-1 in colorectal adenocarcinoma tissues and its clinical significance

作　　者：武雪亮[1] 王立坤[2] 薛军[1] 杨东东[1] 屈明[1] 郭飞[1] 杨瑞敏[2] 刘博[3] 

机构地区：[1]河北北方学院附属第一医院血管腺体外科,张家口075000 [2]河北北方学院附属第一医院超声医学科 [3]河北北方学院附属第一医院病理科

出　　处：《山西医科大学学报》2016年第3期228-231,共4页Journal of Shanxi Medical University

基　　金：河北省卫计委医学科学研究重点课题计划资助项目(20150058);河北省科技支撑计划资助项目(152777237);河北省张家口市科技局指令性计划资助项目(1311055D)

摘　　要：目的研究DNA结合分化抑制蛋白1(Id-1)在结直肠癌组织中的表达及其与临床病理参数间的相关性。方法选取我院结直肠癌和正常黏膜组织各50例,应用逆转录聚合酶链反应法(RT-PCR)和蛋白质印迹法(Western blot)检测两组中Id-1 mRNA和蛋白质的表达情况,并应用免疫组化法(SP)检测Id-1在两组中的表达,分析Id-1在结直肠肿瘤发生、发展中的作用。结果结直肠癌组织中Id-1 mRNA的表达水平较正常组织高(0.96±0.03 vs 0.20±0.04,P=0.011);Id-1蛋白在结直肠癌组织中的表达水平亦较正常组织高(0.82±0.04 vs 0.31±0.02,P=0.020)。免疫组化显示Id-1在结直肠癌组织组中阳性表达明显高于正常黏膜中的表达,差异有统计学意义(72.00%vs 24.00%,χ2=23.431,P=0.000)。Id-1表达与肿瘤浆膜浸润、TNM分期、淋巴结转移、肝转移及脉管浸润等密切相关(均P<0.01),而与肿瘤大小及分化程度无关(P>0.05)。结论 Id-1的特异性高表达与结直肠腺癌的发生、发展具有较高的相关性,有望成为特异性较高的肿瘤标志物。Objective To investigate the expression of Id-1 in human colorectal adenocarcinoma tissues and explore their correlation with clinical pathological parameters of colorectal cancer. Methods The expression levels of Id-1 mRNA and protein in 50 specimens of normal colorectal tissues,and 50 specimens of colorectal adenocarcinoma tissues were detected using reverse-transcription polymer ase chain reaction and Western blot. Meanwhile Id-1 protein was detected using immunohistochemistry. The correlation between Id-1expression and the clinical pathological features was analyzed. Results The expression level of Id-1 mRNA in colorectal adenocarci noma tissues and normal colorectal tissues were 0. 96 ± 0. 03 and 0. 20 ± 0. 04,and there was significant difference（ P = 0. 011）. The expression of Id-1 protein in colorectal adenocarcinoma tissues was higher than that in normal colorectal tissues（ 0. 82 ± 0. 04 vs 0. 31 ±0. 02,P = 0. 020）. The positive expression rate of Id-1 protein in colorectal adenocarcinoma tissues was higher than that in normal color ectal tissues（ 72. 00% vs 24. 00%,χ^2= 23. 431,P = 0. 000）. Expression of Id-1 was correlated with the depth of tumor invasion,TNM stage,lymph node metastasis,vessel invasion,and liver metastasis（ P〈0. 01）,but not with the tumor size and differentiation degrees（ P〈0. 05）. Conclusion The high expression of Id-1 in colorectal adenocarcinoma tissues plays an important role in the process of cancer,and is expected to become a new tumor monitoring indicator for clinical diagnosis and treatment and prognosis judgment.

关 键 词：DNA结合分化抑制蛋白1 结直肠肿瘤 逆转录聚合酶链反应 WESTERN BLOT 免疫组化 

分 类 号：R735.37[医药卫生—肿瘤]