DNA倍体分析在口腔癌及癌前病变中的应用  

DNA ploidy analysis in oral cancer and precancerous lesions

作　　者：宁毅[1] 范永锋[2] 孙睿[3] 

机构地区：[1]山西医科大学口腔医学系,太原030001 [2]山西医科大学附属人民医院口腔内科 [3]山西医科大学附属人民医院口腔颌面外科

出　　处：《中国药物与临床》2016年第2期161-163,共3页Chinese Remedies & Clinics

基　　金：山西省自然科学基金(2011011037)

摘　　要：目的评价和比较脱落细胞DNA倍体分析与甲苯胺蓝染色两种方法对诊断癌前病变和口腔癌的应用价值。方法收集山西省人民医院口腔外科门诊及住院患者220例,分组应用DNA倍体分析和甲苯胺蓝染色两种检查方法检测。结果 DNA倍体分析对癌前病变和恶性病灶都显示出高特异性和灵敏度;而甲苯胺蓝对癌前病变和恶性病灶染色结果显示出中等灵敏度和低特异性。结论对于口腔癌及癌前病变的诊断,脱落细胞DNA倍体分析在灵敏度和特异度方面都优于甲苯胺蓝染色。Objective To evaluate and compare the value of exfoliated cell DNA ploidy analysis and toluidine blue staining in the diagnosis of precancerous lesions and oral cancer. Methods A total of 220 outpatients and inpatients hospitalized in the Department of Oral Surgery, Shanxi Provincial People ′s Hospital, were included in the study.All patients were grouped and examined by DNA ploidy analysis and toluidine blue staining. Results DNA ploidy analysis showed high specificity and sensitivity for precancerous lesions and malignant lesions. Toluidine blue staining showed moderate sensitivity and low specificity for precancerous lesions and malignant lesions. Conclusion For the diagnosis of oral cancer and precancerous lesions, exfoliated cells-DNA ploidy analysis is better than toluidine blue staining in sensitivity and specificity.

关 键 词：口腔肿瘤 早期诊断 甲苯胺蓝染色 DNA倍体分析 

分 类 号：R739.85[医药卫生—肿瘤]