羧基水溶性量子点在鼻咽癌标志物EBNA1标记中的应用  

Application of carboxyl water-soluble quantum dots in nasopharyngeal carcinoma marker EBNA1 labeling

作　　者：何剑[1] 吴平[1] 徐婧[1] 米日喀米力.玉苏甫[1] 廖玲凤[1] 全华涛[1] 张帅[1] 田勇泉[1] 唐瑶云[1] 

机构地区：[1]中南大学湘雅医院耳鼻咽喉头颈外科/耳鼻咽喉重大疾病湖南省重点实验室,长沙410008

出　　处：《检验医学与临床》2016年第6期721-723,共3页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81170923)

摘　　要：目的探讨605nm羧基水溶性量子点在鼻咽癌标志物EB病毒核抗原1(EBNA1)中的标记方法。方法将605nm羧基水溶性量子点和鼻咽癌EBNA1在1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐的作用下共价交联,生成量子点标记的抗原,对标记后的抗原采用紫外可见吸收谱、荧光光谱测定,琼脂糖凝胶电泳分析。结果羧基水溶性量子点与鼻咽癌EBNA1抗原通过表面氨基与羧基缩合形成的稳定共价键,羧基水溶性量子点与鼻咽癌EBNA1抗原之间实现了成功连接,标记后荧光发射光谱检测结果表明,标记后的量子点EBNA1溶液的发射峰位置在380nm附近,保持良好的荧光特性。结论 605nm羧基水溶性量子点采用共价交联法可稳定标记鼻咽癌标志物EBNA1,为后续研究提供可靠依据。Objective To explore the marking method of 605 nm carboxyl water-soluble quantum dots in nasopharyngeal carcinoma marker EBNA1.Methods The 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen were performed the covalent cross-linking under the action of 1-（3-dimethyl amino propyl）-3-2ethyl carbon imine hydrochloride,the quantum dots labeled antigen was generated,then the labeled antigen was detected by UV-Vis absorption spectroscopy,fluorescence spectrometry and agarose gel electrophoresis analysis.Results The stable covalent bond formation of carboxyl water-soluble quantum dots with nasopharyngeal carcinoma EBNA1 antigen by the surface amino and carboxyl condensation on was found,the carboxyl water-soluble quantum dots achieved the successful connection with EBNA1 nasopharyngeal carcinoma antigen,the labeled fluorescence emission spectrum detection results showed that the emission peak position of the labeled quantum dots EBNA1 solution was near 380 nm,keeping good fluorescence properties.Conclusion The 605 nm carboxyl water-soluble quantum dots can stably label nasopharyngeal carcinoma marker EBNA1 by adopting the covalent cross-linking method to provide reliable basis for subsequent research.

关 键 词：羧基水溶性量子点 鼻咽癌 EBNA1 共价交联法 

分 类 号：R730.2[医药卫生—肿瘤]