meteorin蛋白促进氧糖剥夺后星形胶质细胞的存活  

Neuroprotection of meteorin against oxygen-glucose deprivation-induced injury in cultured astrocytes

作　　者：陈嘉龙[1] 许丽丽[1] 谢怡[1] 王兆露[2] 叶瑞东[1] 刘新峰[1] 

机构地区：[1]南京大学医学院附属金陵医院(南京军区南京总医院)神经内科,南京210002 [2]江苏省人民医院神经内科,南京210029

出　　处：《医学研究生学报》2016年第3期235-239,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81200892,31300900,81471182,81400332)

摘　　要：目的 meteorin在脑梗死后的表达和影响尚不明确。文中旨在探讨meteorin在局灶性脑缺血再灌注(middle cerebral artery occlusion,MCAO)模型的表达和定位,以及通过建立体外氧糖剥夺(oxygen-glucose deprivation,OGD)模型,观察meteorin对神经元、星形胶质细胞OGD后的影响。方法大鼠按随机数表法随机分为6组:假手术组,再灌注后6 h、12 h、24h、3 d、7 d组。建立大鼠MCAO模型,取皮层脑组织行免疫印迹检测meteorin的表达,用免疫组化的方法检测meteorin的细胞定位。建立体外OGD模型,将原代大鼠神经元分为对照A组、OGD A组、OGD+重组meteorin蛋白A组、OGD+PBS A组;将原代大鼠星形胶质细胞分为对照B组、OGD B组、OGD+重组meteorin蛋白B组、OGD+PBS B组。用免疫细胞化学的方法检测meteorin的表达。用CCK-8染色检测其细胞活性。结果 meteorin在MCAO模型后表达下调,3 d时表达量最低(P<0.01),主要表达于神经元和星形胶质细胞中。meteorin作用于OGD损伤的神经元后,与对照A组A值(1.51±0.06)比较,OGD A组、OGD+meteorin A组、OGD+PBS A组的A值减小(0.63±0.09、0.78±0.22、0.60±0.09),差异有统计学意义(P<0.01);但OGD+meteorin A组、OGD A组、OGD+PBS A组差异无统计学意义(P>0.05)。给予meteorin干预后对OGD引起星形胶质细胞损伤的影响,与对照B组A值(2.43±0.12)比较,OGD B组、OGD+meteorin B组、OGD+PBS B组的A值(1.24±0.17,1.51±0.30,1.23±0.16)减小(P<0.01);OGD B组、OGD+PBS B组A值亦较OGD+meteorin B组减小(P<0.05)。结论 meteorin在局灶性脑缺血再灌注后主要表达在神经元星形胶质细胞中,给予外源性重组meteorin可促进OGD后星形胶质细胞的存活。Objective The expression and neuroprotective effect of meteorin in neurons and astrocytes after cerebral infarction have yet to be clarified. This study was to investigate the expression and location of meteorin in the rat model of middle cerebral artery occlusion（ MCAO） and its neuroprotective effect against oxygen-glucose deprivation（ OGD）-induced injury in cultured neurons or astrocytes. Methods Forty-two healthy adult male SD rats were randomly divided into 6 groups of equal number： sham operation and6 h,12 h,24 h,3 d,and 7 d MCAO. The cortical tissue was harvested for determination of the expression and location of meteorin by Western blot and immunohistochemistry as well as the meteorin expression in the neurons and astrocytes subjected to OGD. The neuroprotective effect of meteorin on the neurons and astrocytes was evaluated by CCK8 and PI / Hoechst33342 staining. Results Compared with the sham operation group,the expression of meteorin was decreased after MCAO and reached the lowest level at 3 days（ P 0. 01）,mainly in the neurons and astrocytes. Exogenous meteorin helped the survival of the astrocytes subjected to OGD,with the A value decreased in the groups of OGD（ 0. 63）,OGD ＋ meteorin（ 0. 78）,and OGD ＋ PBS（ 0. 60） as compared with the sham control（ 1. 51）（ P 0. 01）,with no statistically significant differences among the former three groups（ P 0. 05）. Concerning the effect of meteorin intervention on the OGD-induced injury of the astrocytes,the A value was significantly reduced in OGD（ 1. 24 ± 0. 17）,OGD ＋ meteorin（ 1. 51 ± 0. 30）,and OGD ＋ PBS（ 1. 23 ± 0. 16） in comparison with the sham control（ 2. 43 ± 0. 12）（ P 0. 01）,lower in the OGD and OGD ＋ PBS groups than in the OGD ＋ meteorin group（ P 0. 05）. Conclusion Meteorin is mainly expressed in the neurons and astrocytes after MCAO and it promotes the survival of the astrocytes with OGD-induced injury.

关 键 词：meteorin 氧糖剥夺 局灶性脑缺血再灌注模型 星形胶质细胞 神经元 

分 类 号：R743[医药卫生—神经病学与精神病学]