辽宁省5种老鹳草中5种活性成分的含量测定  

Content Determination on Five Active Ingredients in Five Species Geranii Herba of Liaoning Provience

作　　者：谷静逸 尹海波[1] 赵容[1] 贾晓晴[1] 邵飞[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国实验方剂学杂志》2016年第6期26-29,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省自然科学基金项目(201402004);辽宁中医药大学杏林学者青蓝工程杰出青年基金(50213);中医药行业科研专项(201407002)

摘　　要：目的：建立HPLC同时测定老鹳草属5种老鹳草中原儿茶酸、没食子酸、柯里拉京、鞣花酸和金丝桃苷含量的方法。方法：采用Agilent TC-C18色谱柱（4.6 mm×250 mm,5μm）,流动相0.1%磷酸-乙腈梯度洗脱,流速1.0 m L·min-1,检测波长280 nm,柱温30℃。结果：原儿茶酸、没食子酸、柯里拉京、鞣花酸和金丝桃苷5种成分的线性范围分别为0.500~5.000μg（r=0.999 5）,0.075~0.750μg（r=0.999 7）,1.790~17.900μg（r=0.999 9）,0.580~5.800μg（r=0.999 6）,0.065~0.650μg（r=0.999 6）,平均回收率在94.05%~102.58%,RSD在1.6%~2.9%。结论：该方法简单、准确,具有良好的重复性和稳定性,可为老鹳草药材的质量评价提供一定的参考依据。Objective： To establish an HPLC method to determine the content of protocatechuic acid,gallic acid, corilagin,ellagic acid and hyperoside in Geranii Herba. Method： HPLC column Agilent TC-C18（ 4. 6 mm × 250 mm,5 μm） was eluted with the,mobile phase of 0. 1% phosphonic acid-acctonitril in a gradient mode,the flow rate was 1. 0 m L·min- 1,the detection wave-length was 280 nm,and the temperature was 30 ℃.Result： The linear ranges of the 5 ingredients in HPLC were 0. 500-5. 000 μg（ r = 0. 999 5）,0. 075-0. 750 μg（ r = 0. 999 7）,1. 790-17. 900 μg（ r = 0. 999 9）,0. 580-5. 800 μg（ r = 0. 999 6）,0. 065-0. 650 μg（ r =0. 999 6）,the ranges of average recoveries and RSD were 94. 05%-102. 58% and 1. 6%-2. 9%. Conclusion：The method is simple and accurate with a high repeatability,which could provide reference for the quality evaluation of Geranii Herba.

关 键 词：老鹳草 原儿茶酸 没食子酸 柯里拉京 鞣花酸 金丝桃苷 

分 类 号：R284.1[医药卫生—中药学]