青蒿琥酯对口腔扁平苔藓患者外周血T-bet和GATA-3平衡的影响  

The influence of artesunate on T-bet/GATA-3 equilibrium of peripheral blood in oral lichen planus

作　　者：刘立访[1] 黄宇蕾[2] 邓雪莲[1] 于春梅[1] 

机构地区：[1]佛山市第一人民医院口腔科,广东佛山528000 [2]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室

出　　处：《口腔疾病防治》2016年第4期222-225,共4页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金青年科学基金(81500865);佛山市卫生局医学科学立项课题(2013027)

摘　　要：目的探析青蒿琥酯对口腔扁平苔藓(oral lichen planus,OLP)患者外周血转录因子T-bet和GATA-3平衡的影响作用。方法以18例口腔扁平苔藓患者作为研究对象,采取密度梯度离心法对其外周血单核细胞(peripheral blood mononuclear cells,PBMCs)进行分离、培养,每例患者的PBMCs样本均分为4组,对照组不加任何药物,碳酸氢钠组加入质量分数5%的碳酸氢钠溶液,低浓度组加入终浓度为1μmol/L的青蒿琥酯溶液,高浓度组加入终浓度为100μmol/L的青蒿琥酯溶液。应用逆转录聚合酶链反应技术和免疫印迹法分别检测培养细胞中T-bet和GATA-3的mRNA和蛋白的表达水平。结果对照组和碳酸氢钠组培养细胞中T-bet、GATA-3和T-bet/GATA-3的mRNA及蛋白表达水平差异无统计学意义(P>0.05);低浓度组和高浓度组T-bet/GATA-3的mRNA及蛋白表达水平均高于对照组(P<0.05),且高浓度组T-bet/GATA-3的mRNA及蛋白表达水平明显高于低浓度组(P<0.05)。结论青蒿琥酯能有效调节OLP患者外周血内T-bet和GATA-3的失衡状态,本研究结果为青蒿琥酯用于OLP的临床治疗提供了理论依据。Objective To explore influence of artesunate on T-bet /GATA-3 equilibrium of peripheral blood in oral lichen planus. Methods 18 OLP patients were selected,and peripheral blood mononuclear cells（ PBMCs） were isolated by density gradient centrifugation. The cells were cultured and treated with different concentrations（ 0, 1, 100 μmol / L）of artesunate. Reverse transcription polymerase chain reaction（ RT-PCR） and Western blot were applied to detect the T-bet and GATA-3 mRNA and protein expression respectively. Results The T-bet / GATA-3 mRNA and protein expressions in groups treated with artesunate（ 1 μmol / L 100 μmol / L） were higher than that of control group（ treated without artesunate）（ P〈0. 05）, and the expressions were improved significantly in high concentration group（ 100 μmol / L） compared with low concentration group（ 1 μmol / L）（ P〈0. 05）. Conclusion Artesunate can regulate the T-bet / GATA-3 balance in OLP effectively.

关 键 词：青蒿琥酯 口腔扁平苔藓 T-BET GATA-3 逆转录聚合酶链反应 

分 类 号：R781.5[医药卫生—口腔医学]