急性脑梗死超早期血液生物标志物β-actin的表达及临床意义  

Expression and clinical significance of ultra early stage biomarker blood β-actin in patient with acute cerebral infarction

作　　者：张亚萍[1] 王斌达[1] 刘春艳[1] 叶指南 唐维国[1] 吴舟娜[1] 何松彬[1] 

机构地区：[1]温州医科大学附属舟山医院神经内科,浙江舟山316000

出　　处：《温州医科大学学报》2016年第3期174-177,共4页Journal of Wenzhou Medical University

基　　金：浙江省自然科学基金资助项目(Y2111264);浙江省医药卫生一般研究计划项目(2012KYB224);舟山市科技计划项目(10141)

摘　　要：目的:探讨急性脑梗死超早期(发病<6 h)血液β-actin的表达状态及临床意义。方法:收集急性脑梗死患者80例,设立为急性脑梗死组,并且按不同发病时段,分为脑梗死2 h组、脑梗死4 h组、脑梗死6 h组、脑梗死24 h组及脑梗死72 h组,每组16例;并且与非脑梗死组进行对照比较。非脑梗死组包括短暂性脑缺血发作组、脑出血组及健康人群组,每组16例。采用酶联免疫吸附法检测血液β-actin的表达状态,并与临床病情结合进行分析。结果:1急性脑梗死组的血液β-actin含量为(4.24±2.74)ng/mL,明显高于非脑梗死组的(2.25±1.65)ng/mL,差异有统计学意义(P<0.05);其中,脑梗死2 h组血液β-actin含量与非脑梗死人群各组比较差异有统计学意义(P<0.05);2通过ROC曲线确定血液生物标志物β-actin早期诊断急性脑梗死的截断值为2.10 ng/mL时,具有较高的灵敏度和一定的特异度;3急性脑梗死组血液β-actin水平与梗死面积大小以及NIHSS评分呈一定相关性。结论:急性脑梗死超早期血液β-actin的表达升高可能是一种实用的血清生物标志物。Objective： To explore the expression level of blood β-actin and its clinical significance in ultra early stage of acute cerebral infarction. Methods： Eighty patients with acute cerebral infarction（ACI） were selected as cerebral infarction group, and they were divided into five subgroups by different periods（2 hours, 4 hours, 6 hours, 24 hours and 72 hours）, 16 patients in each subgroup. Other 48 cases without cerebral infarction were selected as control group, including 16 cases of transient ischemic attack, 16 cases of cerebral hemorrhage and 16 cases of healthy contrast group. The content of blood β-actin was detected by enzyme-linked immunosorbent method and analyzed combined with clinical condition. Results： ①The content of blood β-actin in the cerebral infarction group was significantly higher than those in the control group（P〈0.05）. ②The diagnostic kit for ACI was determined at 2.10 ng/m L by ROC curve. It had high sensitivity and specificity.③The level of blood β-actin in patients with acute cerebral infarction has certain correlation with the area of cerebra1 infarction as well as NIHSS scores. Conclusion： The elevated level of blood β-actin may be a practical biomarker of acute cerebral infarction in ultra early stage.

关 键 词：急性脑梗死 超早期 Β-ACTIN 生物标志物 

分 类 号：R743.33[医药卫生—神经病学与精神病学]