结直肠癌患者肿瘤组织及血清ADAM10的表达  

Study on the expression of ADAM10 in colorectal cancer tissues and serum

作　　者：郑佳[1] 盛显仓 

机构地区：[1]台州市第一人民医院消化内科,浙江台州318020

出　　处：《温州医科大学学报》2016年第3期190-193,198,共5页Journal of Wenzhou Medical University

基　　金：台州市黄岩区科技计划项目(2011062-3)

摘　　要：目的:检测结直肠癌患者肿瘤组织和血清中解离素金属蛋白酶10(ADAM10)的表达,分析其与结直肠癌发生、发展及临床病理特征间的联系。方法:应用免疫组织化学SP法检测59例结直肠癌组织、30例结直肠息肉组织及30例正常对照人群肠黏膜中的ADAM10的表达情况,采用双抗体夹心ELISA方法检测上述3组人群血清中的ADAM10表达水平。结果:结直肠癌患者组织和血清中ADAM10的表达水平显著高于结直肠息肉组和正常对照人群(P<0.001);伴随着肿瘤Duke分期的增加,结直肠癌患者组织和血清中ADAM10表达水平进行性升高。癌组织和血清中的ADAM10表达水平和结直肠癌患者的性别、年龄、肿瘤部位、肿瘤大小、组织类型无关(P>0.05),而与肿瘤的分化程度、淋巴结转移、肿瘤远处转移、Duke分期有关(P<0.01)。结论:ADAM10在结直肠癌患者癌组织和血清中高表达,且和结直肠癌的重要临床病理特征相关,在结直肠癌的诊断中具有一定的临床应用价值。Objective： To detect the expression of ADAM10 in colorectal cancer tissue and serum, analyze the relationship between colorectal cancer occurrence, development, and clinical pathological characteristics. Methods： Samples of 59 colorectal cancer tissues, 30 colorectal polyps tissues and 30 health human of normal intestinal mucosa tissue were collected to detect the expressions of ADAM10 with immunohistochemistry SP. ADAM10 expression in serum of the above three groups was detected with double antibody sandwich ELISA method. Results： The expression level of ADAM10 in serum and tissue in the colorectal cancer patients was significantly higher than that in colorectal polyps groups and normal control groups（P〈0.001）. ADAM10 expression levels were elevated in serum and tissue of the colorectal cancer patients with the increase of tumor Duke stage. The expression level of ADAM10 in serum and tissue of the colorectal cancer patients was no relationship with the sex, age, the location of the tumor, tumor size, histological type（P〈0.05）, and was correlated with the tumor differentiation, lymphoid node diversion, distant metastasize, Duke stage（P〈0.01）. Conclusion： Colorectal cancer patients has higher expression of ADAM10 in cancer tissue and serum, and related with clinical pathological characteristics of colorectal cancer. It has a certain clinical value in the diagnosis of colorectal cancer.

关 键 词：结直肠肿瘤 病理分析 解离素金属蛋白酶10 

分 类 号：R735.3[医药卫生—肿瘤]