NHERF表达对宫颈癌细胞耐药性的影响  

Effects of NHERF expression on drug resistance in cervical cancer cells

作　　者：施文[1,2,3] 陶涛[1] 杨晓梅[1] 贺俊崎[1] 

机构地区：[1]首都医科大学基础医学院生物化学与分子生物学系,北京100069 [2]首都医科大学附属北京友谊医院科研实验中心,北京100050 [3]移植耐受与器官保护北京市重点实验室,北京100050

出　　处：《临床和实验医学杂志》2016年第6期523-526,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金项目(81272887;81472409)

摘　　要：目的探讨钠氢交换调控因子(NHERF)表达对宫颈癌细胞耐药性的影响。方法检测顺铂处理下He La细胞NHERF表达量的差异。干扰He La细胞中NHERF的表达,检测He La细胞在顺铂处理下细胞活力的变化。并在NHERF表达减少的情况下,检测顺铂、紫杉醇及甲氨喋呤处理对He La细胞凋亡的影响。结果顺铂处理下He La细胞中NHERF表达量下降。干扰NHERF表达的He La细胞耐顺铂活力增加。顺铂及紫杉醇处理组中,NHERF表达下降可以减少细胞凋亡。结论 NHERF表达下降可以增加宫颈癌细胞的多药耐药性。Objective The effects of NHERF expression on drug resistance in hela cells were investigated. Methods Expression of NHERF were detected in hela cells dealed with different concentrations of cisplatin. The effects of NHERF knock down on the viability of cisplatin resistant hela cells were detected. Therefore,the effects of cisplatin,taxol and methotrexate on apoptosis of NHERF knocked down He La cells were determined. Results Down- regulation of NHERF was detected in hela cells dealed with high concentrations of cisplatin. The cell viability was increased in NHERF knocked down hela cells dealed with cisplatin. The apoptosis of NHERF knocked down hela cells dealed with cisplatin and taxol were decreased. Conclusion Down- regulation of NHERF can increase the multiple drug resistance of cervical cancer cells.

关 键 词：宫颈癌 细胞耐药性 NHERF 

分 类 号：R737.33[医药卫生—肿瘤]