上皮间质转化相关蛋白E-cadherin、N-cadherin及Snail在子宫内膜异位症相关卵巢癌的表达及意义  

Expression and Significance of Epithelial-Mesenchymal Transformation Related Protein E-cadherin,N-cadherin and Snail in Endometriosis-Associated Ovarian Cancer

作　　者：马平川[1] 何宏月 王芳[1] 范晓东[2] 林琬君[2] 朱颖军[2] 

机构地区：[1]天津医科大学研究生院,天津300070 [2]天津市中心妇产科医院,天津300100

出　　处：《实用妇产科杂志》2016年第3期211-215,共5页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:观察上皮间质转化(EMT)相关蛋白E-cadherin、N-cadherin及Snail在子宫内膜异位症相关卵巢癌(EAOC)的表达情况,探讨其与EAOC的关系。方法:应用免疫组织化学方法(SP法)检测30例EAOC组织、30例单纯卵巢子宫内膜异位症(EMs)组织中E-cadherin、N-cadherin、及Snail蛋白的表达情况。结果:E-cadherin、N-cadherin及Snail蛋白在EAOC组织中的阳性表达率分别为30.0%、83.3%、90.0%,在EMs组织中的阳性表达率分别为76.7%、33.3%、40.0%,差异均有统计学意义(P<0.05)。在EAOC组织中,E-cadherin蛋白呈低表达,N-cadherin及Snail蛋白呈高表达,E-cadherin、Ncadherin及Snail蛋白表达与患者年龄、肿瘤直径、病理类型均无关(P>0.05),E-cadherin、N-cadherin与临床分期、淋巴结转移有关(P<0.05),Snail与临床分期有关(P<0.05)。Spearman相关性检验示,E-cadherin蛋白与N-cadherin、Snail蛋白在EAOC中的表达水平呈负相关(r=-0.416,P<0.05;r=-0.457,P<0.05),Snail蛋白与N-cadherin蛋白在EAOC中的表达水平呈正相关(r=0.667,P<0.05)。结论:E-cadherin、N-cadherin及Snail蛋白均参与EAOC病变的进展,Snail可能通过调控E-cadherin、Ncadherin蛋白的表达,进而调控异位子宫内膜的上皮间质转化过程,促进EAOC的发生及转移。Objective： To investigate the expression of epithelial-mesenchymal transformation（ EMT） related protein E-cadherin,N-cadherin and Snail in endometriosis-associated ovarian cancer（ EAOC）,analysis the correlation between EMT and the clinical features of EAOC. M ethods： Using immunohistochemistry（ SP） to examine E-cadherin、N-cadherin and Snail in 30 cases of EAOC and ovarian endometriosis（ EMs）,respectively. Results：The expression rate of E-cadherin、N-cadherin and Snail in EAOC was 30. 0%,83. 3% and 90. 0%,in EMs was76. 7%,33. 3% and 40. 0%（ P 〈0. 05）. The high expression of N-cadherin and the low expression of E-cadherin in EAOC were associated with its clinical stage and lymph node metastasis（ P 〈0. 05）,but not age,tumor size and histological type. Higher expression levels of Snail were associated with clinical stage（ P 〈0. 05） negative correlation existed between expression of E-cadherin and N-cadherin、Snail in EAOC（ r =- 0. 416,P 〈0. 05; r =-0. 457,P 〈0. 05）,while positive correlation existed between expression of N-cadherin and Snail（ r = 0. 667,P 〈0. 05）. Conclusions： E-cadherin、N-cadherin and Snail take part in the progression and development of EAOC,Snail may enhance the EMT of endometriosis through regulating the expression of E-cadherin and N-cadherin and promote the development and metastasis of EAOC.

关 键 词：子宫内膜异位症相关卵巢癌 上皮间质转化 E-CADHERIN蛋白 N-cadherin蛋白 SNAIL蛋白 

分 类 号：R737.31[医药卫生—肿瘤]