破壁灵芝孢子粉对长期镉暴露大鼠金属硫蛋白表达的影响  

Effect of sporoderm-broken Ganoderma lucidum spore powder on metallothionein expression in rats with long-term cadmium exposure

作　　者：葛振丹 黄厚今[1] 朱宝石 冯阳 杨城[3] 

机构地区：[1]遵义医学院公共卫生学院毒理学教研室,贵州遵义563099 [2]黑龙江省伊春市第一医院 [3]遵义医学院

出　　处：《实用预防医学》2016年第4期395-398,共4页Practical Preventive Medicine

基　　金：贵州省科技厅联合基金重点科研项目(黔科合J字LKZ[2013]05号)

摘　　要：目的研究破壁灵芝孢子粉(GLS)对长期镉暴露大鼠金属硫蛋白(MT)基因表达的影响。方法雄性SD大鼠80只,随机分为4组,每组20只,即正常对照组(C)、单独染镉(Cd)和2个给药组(灵芝孢子粉0.5 g/kg和1.0 g/kg+Cd Cl2,即GCd_L和GCd_H组)。除C组外,所有大鼠隔天腹腔注射Cd Cl2(2 mg/kg),GCd_L和GCd_H组每天灌胃灵芝孢子粉混悬液,C组和Cd组大鼠灌胃等体积生理盐水。实验过程观察动物一般表现并定期称重,分别于30、60和90 d处死取肝组织,原子吸收光谱法(AAS)检测90 d肝组织中各亚细胞细胞核、线粒体、微粒体、细胞质Cd含量;采用real time RTPCR法检测MT-1mRNA、MT-2mRNA基因表达。结果与单独染镉组比较,对照组与给药组(GCd)体重差异有统计学意义(P<0.05);灵芝孢子粉使镉在肝组织中各亚细胞的分布发生显著变化(P<0.05);与对照组比较,灵芝孢子粉可诱导镉中毒大鼠MT-1mRNA、MT-2mRNA基因表达上调(P<0.05),且呈时间、剂量效应。结论灵芝孢子粉对长期镉暴露大鼠肝组织有保护作用,其机制可能与调节MT-1mRNA、MT-2mRNA基因表达水平有关。Objective To explore the effect of sporoderm broken Ganoderma lucidum spore （GLS） powder on metallothionein expression in rats with long - term cadmittrn exposure. Methods Eighty male SD rats were randomly divided into 4 groups （each n = 20） ： normal control group, CdCl2 group, 0.5 g/kg GLS plus CdCl2 group （CdCl2） and 1.0 g/kg GLS plus CdCl2 group （GCdH）. Rats of each group were given intraperitioneal injection of 2 mg/kg cadmium chloride （CdCl2） once every two days except for the normal control group. The rats in the GCdL and GCdH groups were respectively orally administered with 0.5 g/kg and 1.0 g/kg GLS once a day, while the rats in the normal control group and CdCl2 group were gavaged with isovolumetric distilled water. The rats＇ manifestation was observed during the experiment and their weight was measured regularly and their hepatic tissues were collected on the 30th, 60th and 90th days. The Cd content in subcellular nucleus, miochondria, microsome and cytoplasm of in the hepatic tissue was tested by atomic absorption spectrophotometer on the 90th day. MT - 1mRNA and MT - 2mRNA in the hepatic tissue were determined by real - time RT - PCR. Results Compared with the rats in the CdCl2 group, the rats＇ weight in the normal control group, CdCl2 group and GCdH group were statistically different （P〈0.05）. GLS significanty changed the subcellular distribution of cadmium in the hepatic tissue （ P 〈 0.05 ）. Compared with normal control group, the expression of MT- 1mRNA and MT- 2mRNA in the GCdL group and GCdH group was up -- regulated in a timedose effect manner （P 〈 0.05）. Conclusions GLS can protect rats from hepatic injury induced by longterm cadmium exposure, and one of the underlying mechanisms may be related to MT - 1mRNA and MT- 2mRNA expression.

关 键 词：灵芝孢子粉 大鼠肝组织 CDCL2 金属硫蛋白 MT-1 MRNA MT-2 MRNA 

分 类 号：R-332[医药卫生]