银耳提取物抗抑郁活性研究  

Anti-depression effect of Tremella fuciformis extract

作　　者：金亚香[1] 张研[2] 刘天戟[1] 

机构地区：[1]吉林大学中日联谊医院,吉林长春130033 [2]吉林大学第二临床医院

出　　处：《实用预防医学》2016年第4期490-493,共4页Practical Preventive Medicine

摘　　要：目的研究银耳提取物对大鼠抗抑郁活性的影响。方法采用雄性Wistar大鼠（体重180-220g）建立慢性应激抑郁症大鼠模型，建模成功大鼠随机分为4组，分别为模型组（生理盐水2．0ml／kg），银耳提取物低剂量组（0．5g／kg），银耳提取物中剂量组（1．0g／kg），银耳提取物高剂量组（1．5g／kg），取空白未建模大鼠作为空白组，给予等体积的生理盐水，五组大鼠连续灌胃给药28d，观察大鼠悬尾不动时间和游泳不动时间，并测定大鼠血浆及下丘脑神经递质多巴胺（DA）、去甲肾上腺素（NE）含量。结果1．5g／kg银耳提取物可以显著提高抑郁大鼠体重（P〈0．01），增加大鼠蔗糖偏爱度（P〈0．01），同时1．0～1．5g／kg银耳提取物剂量依赖性的减少大鼠悬尾不动时间和游泳不动时间（P〈0．05），增加大鼠血浆及下丘脑多巴胺（DA）含量（P〈0．05）。1．5g＆g银耳提取物显著提高大鼠血浆及下丘脑去甲肾上腺素（NE）含量（P〈0．01）。结论银耳提取物具有显著的抗抑郁活性，其作用可能与其增加大鼠血浆及下丘脑神经递质含量相关。Objective To study the anti - depressive effect of Tremella fuciforrnis （TF） extract in rats. Method Chronic unpredictable mild stress was used to induce depression in male Wistar rats with the body weight of 180 - 220 g and the depressive rats were randomly divided into four groups： the model group （2.0 ml/kg sterile ,saline） and low- , medium- and high- dose TF extract groups （0.5, 1.0 and 1.5 g/kg TF extract respectively）. Moreover, normal rats treated with 2.0 ml/kg sterile saline were served as the control group. Rats in each group were administrated by gastrogavage for 28 successive days. The immobility time in tail- suspension test and forced swimming test were otaserved, and the concentrations of dopamine （DA） and norepinephrine （NE） in plasma and hypothalamus were determined. Results Administration （28 days） of TF extract （1.5 g/kg） significantly increased the depressive rats＇ body weight （P 〈 0.01 ） and sucrose preference （P 〈 0.01 ）. At the same time, administration （28 days） of TF extract （1.0 - 1.5 g/kg） significantly decreased the rats＇ immobility time in tailsuspension test and forced swimming test （P〈 0, 05）, but significantly increased the concentration of DA in plasma and hypothalamus （P〈 0.05）. Administration （28 days） of TF extract （1.5 g/kg） significantly increased the concentration of NE in plasma and hypothalamus of the rats （P〈 0.05）. Conclusions TF extract significantly displays anti - depressant- like effect, which may be related to its regulation on dopaminergic system.

关 键 词：银耳 慢性应激 抗抑郁 去甲肾上腺素 多巴胺 

分 类 号：R-332[医药卫生]