自体多免疫细胞疗法联合恩替卡韦治疗慢性乙型活动性肝炎的短期疗效观察  

Study on the short-term efficacy of autologous immune cell therapy combined with entecavir in the treatment of chronic hepatitis B

作　　者：俞哲[1] 刘军权[1] 李佳佳[1] 王琛[1] 王飞[1] 李晓鸥[1] 骆欣[1] 

机构地区：[1]杭州市西溪医院杭州市第六人民医院肝病科,浙江杭州310023

出　　处：《中国现代医生》2016年第6期22-25,共4页China Modern Doctor

基　　金：浙江省中医药科学研究基金计划(2012ZB132)

摘　　要：目的探讨多种自体免疫细胞与恩替卡韦联合治疗慢性乙型活动性肝炎的抗病毒疗效。方法将2015年4~9月我院收治的慢性活动性乙型病毒性肝炎患者26例分为两组（对照组和联合治疗组）,两组均予以抗病毒治疗,其中联合治疗组12例接受多细胞免疫疗法联合恩替卡韦治疗,对照组14例单用恩替卡韦治疗。并分离单核细胞,体外培养γδ T细胞、NK细胞、NKT细胞,治疗8~12周后评价其疗效。结果治疗前两组患者在ALT、HBsAg、HBeAg、HBV DNA比较无统计学差异,联合治疗组HBs Ag下降速率（治疗前/治疗后）与对照组比较有统计学差异（4.15±0.86 vs 1.80±0.15,t=2.09,P〈0.05）;ALT、HBV DNA在治疗前后,联合治疗组下降幅度大于对照组,但差异无统计学意义（P〉0.05）;而HBeAg两组对比无显著性差异。联合组不良反应轻微。结论自体多免疫细胞疗法联合恩替卡韦可促进HBsAg清除,对HBV的清除可能有帮助。Objective To evaluate the efficacy of treatment for hepatitis B virus combined entecavir with a variety of autoimmune cells. Methods 26 hospital patients with chronic hepatitis B were divided into two groups（control group and combination treatment group） voluntarily during the period from April to September in 2015. All patients were in accordance with the indications for anti viral treatment. The combination treatment group included 12 patients received treatment combined entecavir with a variety of autoimmune cells and the control group involved 14 patiens accepted treatment of entecavir alone. Mononuclear cells were isolated, γδ T cells, NK cells, NKT cells were cultured in vitro.The efficacy of the two groups were evaluated after 8-12 weeks. Results There were no statistically significant differences in ALT, HBsAg, HBeAg, HBV DNA between the two groups before treatment. There were significant differences in the decrease rate of HBs Ag（before/after treatment） between the two groups（4.15±0.86 vs 1.80±0.15,t=2.09,P〈0.05）; The efficacy of treatment group was greater than the control group in ALT, HBV DNA, but there was no statistically significant difference（P〉0.05）. There was no significant difference between the two groups in the inhibition of HBe Ag. Adverse reactions in the treatment group were mild. Conclusion Multicellular autologous immunotherapy combined with entecavir could promote removal of HBs Ag. Multicellular autologous immunotherapy may be helpful to the removal of HBV.

关 键 词：自体免疫细胞 NK细胞 NKT细胞 ΓΔT细胞 恩替卡韦 慢性乙型病毒性肝炎 

分 类 号：R575.1[医药卫生—消化系统]