盲肠结扎穿孔败血症小鼠模型的手术方式优化  

An improved mice model of cecal ligation and perforation for sepsis

作　　者：郭佳南 刘文钰[1] 周东[1] 田林郁[1] 

机构地区：[1]四川大学华西医院神经内科,四川成都610041

出　　处：《四川生理科学杂志》2016年第1期5-8,共4页Sichuan Journal of Physiological Sciences

基　　金：国家自然科学基金青年科学基金项目(编号:81301659)

摘　　要：目的:探究如何对传统盲肠穿孔结扎(Cecal ligation-and-perforation,CLP)败血症模型手术方式进行优化。方法:选取雄性昆明小鼠103只按是否给予亚胺培南/西司他丁分为:抗生素组、无抗生素组和假手术组。在传统CLP手术方式的基础上缩小手术切口,调整切口位置和术后抗生素用量来观察接受手术小鼠术后存活率及不同批次间小鼠存活率一致性等指标,并与既往文献报道的同类模型进行比较。结果:在本次实验103只小鼠手术中,抗生素组、无抗生素组和假手术组三组小鼠存活时间的分布总体有差异(P=0.026)。组间两两比较发现:抗生素组与无抗生素组7天生存率明显高于无抗生素组(P<0.001),假手术组生存率明显高于无抗生素组生存率(P<0.001),而抗生素组与假手术组存活率差异无统计学意义(P=0.331)。抗生素组10个批次小鼠存活率的Cronbach’sα系数为0.954。结论:优化后的CLP小鼠模型存活率与传统模型相似,具更亦操作的标准化的手术流程,切口更小,组间生存率一致性高,可重复性好。Objective：To explore how to improve traditional Cecal-ligation and puncture（CLP）procedure.Methods：A total of103 KM male mice were randomly divided into three groups：antibiotic group,no antibiotic group and sham group.Antibiotic group were treated with imipenem and cilastatin;for sham group,we only open the peritoneum and then closed without therapeutic use of antibiotics.We designed a shorter incision in a new site below xiphoid,changing the dose of antibiotics after surgery and observe the survival rate after surgery.Results：Generally,among all 103 mice accepted the surgery,the survival rate of antibiotic group,no antibiotic group and sham group were with statistically difference（P=0.026）.Comparison between each groups showed that：the seven days postoperative survival rate was higher in antibiotic group and no antibiotic group（P〈0.001）;survival rate in sham group was higher than that in no antibiotic group（P〈0.001）.However,the survival rate between antibiotic group and sham group has no difference（P=0.331）.Cronbach＇sαcoefficient among 10batches＇ s survival rate in antibiotic group is 0.954.Conclusion：Compared with traditional CLP one,improved model has a similar survival rate and a easy standard surgical procedure with smaller incision and a reliable result.

关 键 词：败血症 动物模型 手术方式 盲肠结扎穿孔 生存率 

分 类 号：R-332[医药卫生] R515.3