2个水稻品种苗期高温胁迫差异表达基因的克隆与表达  

Clone and Expression of Differentially Expressed Gene Related to High Temperature Stress at Seedling Stage in Rice of Two Rice Varieties

作　　者：许锦彪[1] 吴春芳[2] 卞晓春[2] 王玲娟[3] 蔡晓东[3] 汪琴[3] 赵华[3] 曹云英[3,4] 

机构地区：[1]南通大学图书馆,江苏南通226019 [2]江苏沿江地区农业科学研究所,江苏如皋226541 [3]南通大学生命科学学院,江苏南通226019 [4]农业部南方平原玉米观察站,江苏南通226019

出　　处：《种子》2016年第3期10-15,共6页Seed

基　　金：江苏省高校自然科学研究面上项目(编号:13KJB 210005);江苏省农业科技自主创新资金项目(编号:CX(13)5085);农业部南方玉米平原观察站开放课题(编号:NT 201401)

摘　　要：拟克隆cDNA-AFLP技术筛选的1个水稻高温差异表达片段,通过荧光定量PCR技术研究该基因在不同处理下的表达模式。结果表明:该片段为Os07g0620200基因,产物为水稻中的热激蛋白N端的DnaJ结构域。定量分析表明,各处理均促进了该基因的表达,推测该基因是水稻的一个胁迫相关基因。2个品种比较,黄华占中该基因的表达量始终高于双桂1号,表明黄华占比双桂1号在逆境中有更好的耐受性。Using the heat-sensitive variety/line Shuanggui No.1 and heat-resistant variey/line Huanghuazhan as the materials,one differentially expressed fragment was obtained and cloned from cDNA-AFLP analysis under high temperature at the seedlings stage.The expression of this fragment were analyzed by fluorescence quantitative PCR（qRT-PCR）under stress treatment.The results indicated that this differentially expressed fragment was Os07g0620200 by sequence analysis,which protein product was canonical N-terminal domain（DnaJ）of the heat shock proteins in rice.The expression of the gene by qRT-PCR was induced under high temperature,high salt,drought and Spm treatments.These results suggested that the Os07g0620200 gene might be a stress-related gene in rice.Between the two cultivars,the expression level of this gene in heat-tolerant variety Huanghuazhan was higher than that in heat-sensitive Shuanggui No.1,indicating that in comparsion to Shuanggui No.1,Huanghuazhan possesses a greater tolerance and better stress response under adverse circumstances and it was affected less by extreme environmental conditions.

关 键 词：基因表达 高温胁迫 荧光定量PCR 水稻 苗期 

分 类 号：S511[农业科学—作物学]