靶向抑制Rad50提高头颈部鳞癌细胞放疗敏感性的初步研究  

Targeted inhibition of Rad50 increases the radiosensitivity of head and neck squamous cell carcinoma cells:A pilot study

作　　者：郑军[1] 王小明[1] 杜婵媛[1] 张梦琦[1] 薛慧 王可[1] 李刚[1] 孙沫逸[3] 白岫峰[1] 

机构地区：[1]西安交通大学附属口腔医院头颈肿瘤外科,710004 [2]黑龙江省鹤岗市人民医院口腔科 [3]军事口腔医学国家重点实验室,第四军医大学口腔医学院头颈肿瘤外科

出　　处：《实用口腔医学杂志》2016年第2期190-195,共6页Journal of Practical Stomatology

基　　金：2014年西安交通大学基本科研业务费自由探索项目(编号:0818-08143011)

摘　　要：目的:探讨靶向抑制Rad50对头颈部鳞癌细胞系放疗敏感性的影响。方法:用siRNA技术建立并筛选Rad50靶向抑制的Tca8113和OSCC-15稳转细胞系,联合应用单次小剂量放疗(2 Gy)处理细胞,Western blot检测Rad50的表达变化;通过Comet assay检测细胞核DNA的双链断裂情况,细胞克隆形成实验检测细胞存活分数的变化;Telomere FISH检测细胞端粒长度的变化。结果:与单次小剂量放疗(2 Gy)处理组相比较,Rad50靶向抑制联合放疗处理的Tca8113和OSCC-15稳转细胞系,Rad50蛋白表达显著降低,细胞核DNA的双链断裂损伤明显加重,细胞增殖速率明显减慢,细胞核内染色体端粒的长度均明显变短。结论:靶向抑制Rad50可以显著增强Tca8113和OSCC-15细胞的放疗敏感性。Objective： To investigate the effects of Rad50-targeted inhibition on the radiosensitivity of head and neck squamous cell carcinoma cells. Methods： Rad50 in Tca8113 and OSCC-15 cells was down-regulated by siRNA. Then the cells were treated by a small dose of radiation（ 2 Gy）,Western blot was used to detect the expression of Rad50 protein. Comet assay was used to evaluate the DNA double strand breaks（ DSBs）. Colony survival assay was performed to detect the survival rate of the cells. Telomere FISH was performed to assess the telomere length in the cells. Results： Target siRNA inhibited Rad50 protein expression in Tca8113 and OSCC-15 cells.siRNA combined with the radiation of 2 Gy produced more DSBs,decreased the proliferation and shortened the nucleus telomere length of the cells more than radiation treatment alone. Conclusion： The targeted inhibition of Rad50 may increase the radiosensitivity of Tca8113 and OSCC-15 cells.

关 键 词：Rad50 靶向抑制 头颈鳞癌细胞系 放疗敏感性 

分 类 号：R739.91[医药卫生—肿瘤]