碰撞诱导解离和高能碰撞解离方式进行蛋白质组学分析的比较  

Comparison of Collision Induced Dissociation and High Energy Collision Dissociation for Proteomic Analysis

作　　者：王曌[1] 孙伟[1] 

机构地区：[1]中国医学科学院基础医学研究所,北京协和医学院基础学院,北京100005

出　　处：《质谱学报》2016年第2期140-146,共7页Journal of Chinese Mass Spectrometry Society

基　　金：国家重点基础研究发展计划(2013CB530805、2014CBA02005);中国科技部重点基础研究计划(2013FY114100)资助

摘　　要：为了比较碰撞诱导解离(collision induced dissociation,CID)和高能碰撞解离(high energy collision dissociation,HCD)两种离子裂解模式在蛋白质组学分析方面的差异,分别应用这两种模式对牛血清白蛋白和细胞裂解物进行分析。通过比较所鉴定到的多肽和蛋白数目,发现在牛血清白蛋白中,HCD碎裂方法所得的谱图匹配率和多肽Mascot得分均较高,表明其质谱图质量较好。在复杂样品细胞裂解物分析中,CID碎裂方法鉴定到的谱图数、多肽数和蛋白数目均较多,表明该模式的灵敏度较高;HCD碎裂方法所得的谱图匹配率和Mascot得分均较高,表明其质谱图质量较好。因此,这两种模式均可用于大规模蛋白质组学分析,CID模式可提供更高的灵敏度,而HCD模式则可获得更高质量的质谱谱图。To comprehensively compare the difference of collision induced dissociation（CID）and high energy collision dissociation（HCD）,the tryptic digests of bovine serum albumin（BSA）and cell lysates were analyzed by LTQ-Velos Orbitrap.The HCD fragmentation identified more peptides and this mode showed higher MS/MS success rate and higher Mascot score in the tryptic digests of BSA.The results demonstrated that the HCD fragmentation has higher MS/MS spectra quality than CID.But in the tryptic digests of cell lysates,the CID fragmentation identified more MS/MS spectra,peptides and proteins,which indicated that it produces higher sensitivity.The HCD fragmenta-tion shows higher MS/MS success rate and higher Mascot score distribution,demonstrating higher quality of MS/MS spectrum.Therefore,based on these findings,both CID and HCD modes could be used for large-scale proteomic analysis.The CID mode has higher sensitivity,and HCD mode can achieve higher quality of MS/MS spectrum.

关 键 词：质谱 碰撞诱导解离(CID) 高能碰撞解离(HCD) 蛋白质组学分析 

分 类 号：O657.63[理学—分析化学]