大鼠牙源性细胞复合nHAC/PLA支架体内构建牙体组织-骨复合体样结构的研究  

Study on Tooth Tissue-Bone Complex-Type Structure Formed in Vivo by Rat Odontogenic Cells Combined with nHAC/PLA Scaffold

作　　者：赵征[1] 刘洪臣[2] 黄征难[1] 鄂玲玲[2] 杨海青[1] 

机构地区：[1]解放军第401医院口腔科,山东青岛266071 [2]解放军总医院口腔科,北京100853

出　　处：《口腔医学研究》2016年第3期244-248,共5页Journal of Oral Science Research

基　　金：中国博士后科学基金特别资助金(编号:201003774)

摘　　要：目的:探讨不同组合生长因子诱导的大鼠牙源性上皮细胞(rDECs)、间充质细胞(rDMCs)复合nHAC/PLA支架体内形成牙体组织-骨复合体样结构的能力。方法:应用酶消化法和差速消化法分离培养rDECs和rDMCs,免疫荧光染色鉴定其来源。将各组细胞-支架复合物移植裸鼠皮下3月、5月后分别取材,检测移植物形成牙体组织-骨复合体样结构能力。结果:成功分离培养、鉴定大鼠牙源性上皮细胞和间充质细胞。细胞-支架复合物移植后3月,组1、2、3在细胞-支架接触部位均发现类骨质、新生成熟骨和血管形成,并有OCN、OPN的阳性表达。组2促成骨作用最强。对照组4没有新生骨形成,仅有少量胶原纤维和血管。移植后5月,组1、3均形成没有髓腔的牙根样结构,部分区域有DSPP、DMP1、CAP表达。组2形成周边有新生骨、中央有单根牙体样组织,中心为含有牙髓样组织和血管的髓腔样结构,并有DSPP、DMP1、OPN、OCN在髓腔内壁牙本质基质、部分牙髓样组织和血管内的阳性表达,ameloblastin、CAP分别在釉质样、牙骨质样组织中表达阳性。对照组4形成新生骨,OCN强阳性表达,未形成牙体样结构。结论:经TGF-β1+BMP4诱导的rDECs+rDMCs-nHAC/PLA体内移植物促成骨和成牙作用最强,移植后5月形成牙体组织-骨复合体样结构。Objective：To investigate the capability of tooth tissue-bone complex-like structure in vivo formed by rDECs＋rDMCs-nHAC/PLA compounds induced by different growth factors.Methods：Rat dental epithelial cells（rDECs）and rat dental mesenchymal cells（rDMCs）were isolated,cultured and identified.The cell-scaffold compounds were transplanted into athymic mice subcutaneously and taken out after 3or 5 months.The capability of tooth tissue-bone complex-like structure formed by the graftswas detected.Results：BothrDECs and rDMCscould be isolated,cultured and identified.After 3months,the osteoid,new bone and blood vessel were found in group 1to 3.The osteogenesis in group2（TGF-β1＋BMP4）was most powerful.There were only a few collagen fibers and blood vessels in the control（group 4）.After 5months,tooth root-like structure without pulp cavity was seen in group 1and 3.In group 2,new bone and tooth-type tissueswere formed.Ameloblastin and CAP were expressed positively in emailloid and cementoid tissues respectively.In group 4,there was new bone but no tooth-like structure.Conclusion：The most powerful way that affects theosteogenesis and tooth-forming forrDECs＋rDMCsnHAC/PLA grafts is TGF-β1＋BMP4.

关 键 词：大鼠牙源性上皮细胞 大鼠牙源性间充质细胞 纳米羟基磷灰石-胶原/聚乳酸 

分 类 号：R783.1[医药卫生—口腔医学]