正畸力作用下压力侧Wnt10b、RANKL在牙周组织中的表达  

Expression of Wnt10b and RANKL in the Pressure Site of Periodontal Tissue under Orthodontic Force

作　　者：王莹[1] 王兰珠[2] 韩博[2] 吴明明[2] 张苗苗[1] 

机构地区：[1]哈尔滨医科大学附属口腔医院正畸科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150000

出　　处：《口腔医学研究》2016年第3期257-260,共4页Journal of Oral Science Research

基　　金：黑龙江省博士后启动基金(项目编号:LBH-Q11033);哈尔滨医科大学附属第一医院博士基金(项目编号:2009B29)

摘　　要：目的:研究大鼠在正畸力作用下压力侧wnt10b、RANKL在牙周组织中的表达变化。方法:将42只健康雄性SD大鼠随机分为7组,即0h、6h、12h、24h、5d、7d、14d组,使用镍钛拉簧对第一磨牙向近中施加50g正畸力,以0h为对照组,每组处死6只。应用实时定量PCR(RT-PCR)对wnt10b、RANKL的mRNA表达进行检测。结果:Real-time PCR结果显示,与对照组相比,实验组压力侧牙周组织中Wnt10b、RANKL mRNA均有表达,其中wnt10b于加力初期表达受抑制,随后升高,于5d达高峰随后下降,14d恢复至正常水平。RANKL表达随加力时间延长而逐渐升高,7d达到高峰,随后下降。结论:Wnt10b、RANKL参与正畸加力作用下的牙周组织改建。Objective：To investigate the changes of RANKL and wnt10 bin the periodontal tissue during orthodontic tooth movement.Methods：Forty two healthy male SD rats were randomly divided into 7groups according to the application loaded time points of 0h,6h,12 h,24h,5d,7d,and 14 d.The group of 0hserved as control and the Nickel-titanium closed-coils springs were used to produce 50 g power to drag the first molars mesially.Realtime PCR（RT-PCR）technology was used to detect the expression of Wnt10 band RANKL.Results：RANKL and Wnt10 bwere both expressed in the pressure site in comparison with the control group according to the results of the RT-PCR.The expression of wnt10 b mRNA was suppressed at the early stage,then increased,and reached the peak after 5-7days.It returned to the normal level after 14 days.The expression of RANKL elevated at first,then peaked after 5-7days,and decreased.Conclusion：Wnt10band RANKL participated in periodontal tissue remodeling under the action of the orthodontic force.

关 键 词：正畸牙移动 破骨细胞分化 牙周组织改建 Wnt10b RANKL 

分 类 号：R783.5[医药卫生—口腔医学]