双缺陷型毕赤酵母X33突变株的诱变育种  

Mutation Breeding of Double Deficient Mutant Pichia pastoris X33 Strain

作　　者：顾鹏飞[1,2] 李萌[1,2] 朱瑞宇[1,2] 金坚[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学药学院,江苏无锡214122

出　　处：《食品与生物技术学报》2016年第2期129-135,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(81101667);江苏省基础研究计划(自然科学基金)项目(BK2009071)

摘　　要：为促进Pichia pastoris X33α-1,6-甘露糖转移酶(OCH1p)与α-1,3-甘露糖转移酶(ALG3p)双突变菌株(och1 alg3 m X33)作为蛋白质表达宿主菌的应用,为双突变菌株进一步糖基化的研究奠定基础。作者通过紫外诱变和常压室温等离子体(ARTP)诱变的方法来提高双突变菌株的生长速度以及适应能力。经过4轮筛选发现了生长速度提高15%、温度敏感性有所降低、细胞存活率有所提高的双突变菌株。To promote the application of α-1, 6-mannose transferase（OCH1p） and α-1,3-mannose transferase（ALG3p） double mutant strain of Pichia pastoris X33（och1 alg3 m X33） as host for protein expression and to lay the foundation for the further glycosylation of the double mutant strain, this study wants to increase the growth rate as well as the ability to adapt of the double mutant strain through ultraviolet mutagenesis and Atmospheric and Room Temperature Plasma（ARTP） mutagenesis methods. At last, after four rounds of screening, a double mutant strain was found to be 15% increase in growth speed, the decrease in temperature sensitivity, and the improvement in cell survival rate.

关 键 词：糖基化 诱变 生长表型 甘露糖转移酶 

分 类 号：Q93[生物学—微生物学]