有氧运动训练和姜黄素对Ⅱ型糖尿病大鼠肝糖原及糖代谢信号通路的影响  

Effects of aerobic exercise training and curcumin on hepatic glycogen and glucose metabolism signaling pathway of type Ⅱ diabetic rats

作　　者：张丽莉[1] 贾鹏[1] 张东海[1] 

机构地区：[1]廊坊师范学院体育学院,河北廊坊065000

出　　处：《食品工业科技》2016年第7期346-349,共4页Science and Technology of Food Industry

摘　　要：为探讨有氧运动训练和姜黄素对Ⅱ型糖尿病大鼠肝糖原及糖代谢信号通路的影响,将大鼠分为对照组(C组),模型组(D组)、运动组(S组)、姜黄素组(H组)和姜黄素+运动组(S+H组),S组和S+H组大鼠给予8周有氧运动训练,H组和S+H组大鼠给予8周姜黄素灌胃干预,测定其空腹血糖、口服葡萄糖耐量实验(OGTT)后各时点血糖、胰岛素分泌、胰岛素敏感性、肝糖原水平及肝脏葡萄糖代谢及胰岛素信号分子mRNA表达的变化。结果表明:与D组相比,S+H组大鼠OGTT实验后各时刻血糖值均降低(p<0.05);且胰岛素水平、胰岛素敏感指数及肝糖原水平均较S组和H组高(p<0.05);肝脏葡萄糖激酶(GK)、肝细胞葡萄糖载体蛋白-2(GLUT2)、肝脏胰岛素样生长因子1(IGF-1)、胰岛素受体底物1(IRS-1)、胰岛素受体底物2(IRS-2)相对表达量均较S组和H组高(p<0.05),而人葡萄糖激酶调节蛋白(GKRP)则较S组和H组降低(p<0.05),且联合干预效果优于单独干预。结论表明有氧运动和姜黄素可有效降低血糖水平,改善胰岛素抵抗,促进肝糖原合成。In order to investigate the influence of aerobic exercise training and curcumin on glycogen and glucose metabolism signaling pathway of type Ⅱ diabetic rats.All rats were divided into the control group（C group）, model group（ group D） ,exercise group（ S group） ,curcumin group（ H group） and turmeric prime ＋ exercise group（ S ＋ H group）.The rats in S group and S ＋ H group were given aerobic exercise training for 8 weeks,and in H group and S ＋ H group were given curcumin intervention for 8 weeks.Fasting blood glucose, blood glucose at each time point after oral lucose tolerance test（OGTT）, insulin secretion, insulin sensitivity, glycogen levels and expressions of hepatic glucose metabolism and insulin signaling molecule mRNA were detected. The results showed that compared with the D group,the glucose values at each time point after OGTT test in the S ＋ H group were lower （p 〈0.05）. Compared with S and H group,the rats insulin levels,insulin sensitivity index and liver glycogen levels were higher in S ＋ H group（ p 〈0.05）.The relative expressions of glucokinase（GK） ,glucose transporter protein-2 （ GLUT2）, insulin-like growth factor ] （IGF-1 ）, insulin receptor substrate ] （IRS-I ）and Insulin receptor substrate 2 （IRS-2）in S ＋ H group were increased in comparison with S and H group（ p 〈0.05 ）,while glucose regulated protein kinase（GKRP）were reduced （p 〈 0.05 ）.And the effect of combined intervention was better than single intervention.Aerobic exercise and curcumin could effectively reduce blood sugar levels,improve insulin resistance, and promote glycogen synthesis.

关 键 词：Ⅱ型糖尿病 姜黄素 有氧运动 肝糖原 糖代谢 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]