碘化物过量对人神经细胞的损伤作用及机制研究  

Excess Iodide Effects on Autophagy of Human Nerve Cells(SH-SY5Y)

作　　者：赵亮[1] 崔玉山[1] 曾强[1] 王凌之[2] 张斌[2] 侯常春[1] 刘洪亮[2,3] 

机构地区：[1]天津市疾病预防控制中心 [2]天津医科大学 [3]天津市预防医学研究所

出　　处：《环境卫生学杂志》2016年第1期16-19,共4页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：国家自然科学基金项目(81372934;81573107);天津市医药卫生2014年度重点攻关项目(14KG120);天津市卫生计生委科技基金项目(2014KY20)

摘　　要：目的探讨碘化物过量对人神经母细胞瘤细胞(SH-SY5Y)的损伤作用及其机制。方法将体外培养的SH-SY5Y细胞用0(对照)、10、20、30 mmol/L碘化钾(KI)分别染毒处理24 h后,采用CCK-8法、单丹黄酰尸胺(MDC)染色法及Wsetern blot实验检测细胞存活率、自噬囊泡生成以及自噬相关蛋白表达情况。结果与对照组相比较,30 mmol/L碘化物染毒组细胞存活率明显下降(P<0.05),自噬囊泡生成明显增多(P<0.05),自噬相关蛋白Beclin1表达明显升高(P<0.05)。结论一定剂量的碘可降低神经细胞的存活率,诱导细胞自噬,碘化物过量对神经细胞的损伤作用可能与其诱导活化细胞自噬有关。Objectives To investigate the damage of excess iodide on human neuroblastoma cells（ SH-SY5Y）and its related mechanism. Methods SH-SY5 Y cells were treated with potassium iodide（ KI） at the concentrations of 0,10,20 and 30 mmol / L in the medium for 24 hours. The viability of cells,the generation of autophagic vesicles and the expression of Beclin1 were tested by CCK- 8 assay,monodansylcadaverine（ MDC）- staining assay and western blot assay. Results Compared with the control group,the viability of cells in the 30 mmol / L KI group was decreased obviously（ P〈 0. 05）,the generation of autophagic vesicles was increased（ P〈 0. 05） and the expression of Beclin1 protein was higher significantly（ P〈 0. 05）. Conclusions Excess iodide can notablely decrease the viability of SH-SY5 Y cells,increase the generation of autophagic vesicles and increase the expression of Beclin1 protein in SH-SY5 Y cells. These results suggested that the damage of nerve cell induced by excess iodide might be related to autophagy.

关 键 词：碘化物 神经细胞 细胞自噬 

分 类 号：R151.41[医药卫生—营养与食品卫生学]