固相萃取-离子色谱法测定茶叶中的阴离子  

SPE-Ion Chromatographic Determination of Inorganic Anions in Tea

作　　者：郑远利 范伢 穆凯艳[1] 谭伟[1] 杨敏[1] 王红斌[1] 

机构地区：[1]云南民族大学化学与生物技术学院,民族药资源化学国家民委-教育部重点实验室,云南昆明650500

出　　处：《食品研究与开发》2016年第2期110-113,共4页Food Research and Development

基　　金：云南民族大学绿色化学与功能材料研究省创新团队项目(2011HC008);云南省高校绿色化学新能源与材料科技创新团队项目(2011UY09);2012年国家级大学生创新创业训练计划项目(201210691001)

摘　　要：建立了用离子色谱同时测定茶叶中的F^-、Cl^-、NO_2^-、NO_3^-、SO_4^(2-)5种重要阴离子的分析方法。以Ionpac AS11为分离柱、AG11为保护柱、NaOH等浓度洗脱,以普洱绿茶为样品,考察了不同的回流时间,提取液体积,筛目大小对茶叶提取率的影响,其他3种茶叶作为推广。结果表明:该方法回归方程的线性相关系数在0.999 1~0.999 8之间,各组分的检出限在0.015mg/L^0.041 mg/L之间,RSD值<5%,加标回收率在80.6%~113.7%之间。该方法具有快速、灵敏、准确的特点,用于实际茶叶样品中阴离子的分析,结果令人满意。Conditions for separation and detection of F^-,Cl^-,NO_2^-,NO_3^- and SO_4^2- by gradient ion chromatography with suppressed conductivity detection were studied.NaOH solution as mobile phase,the target component in the extract were separated on AS11 column with AG11 as guard column.Puer tea as samples,investigated different reflux time,extract volume,the impact of mesh size on the tea extraction rate.It was found that the correlation coefficients of linear regression equations were above 0.999 1-0.999 8,and the detection limits of those anions were in the range of 0.015 mg/L-0.041 mg/L,RSD%5%,the recoveries of the method were between 80.6%to 113.7%.The method offered fast operation,good reproducibility,high sensitivity and accuracy.The method is used for the actual of anion in the tea samples analysis,the result is satisfactory.

关 键 词：固相萃取 离子色谱法 茶叶 阴离子 

分 类 号：TS272.7[农业科学—茶叶生产加工] O657.7[轻工技术与工程—农产品加工及贮藏工程]