吉西他滨诱导胰腺癌PANC-1细胞耐药与C-IAP2表达关系的研究  

Gemcitabine-induced chemotherapy resistance of PANC-1 pancreatic cancer cell line and its association with C-IAP2 expression

作　　者：张帆[1] 黄鹤光[2] 

机构地区：[1]福建医科大学附属第一医院化疗科,福州350005 [2]福建医科大学附属协和医院基本外科,福州350004

出　　处：《中国临床药理学杂志》2016年第6期540-542,共3页The Chinese Journal of Clinical Pharmacology

基　　金：福建省自然科学基金资助项目(2010J05058)

摘　　要：目的研究吉西他滨(Gem)诱导胰腺癌PANC-1细胞耐药与C-IAP2的关系。方法用间歇浓度递增法诱导胰腺癌细胞株PANC-1对吉西他滨耐药,将胰腺癌细胞株分为亲本株PANC-1组和耐药株PANC-1/Gem组。用四唑盐比色法检测细胞的半抑制浓度(IC_(50))值,用细胞计数法检测细胞倍增时间,用流式细胞术检测细胞周期,用免疫荧光法检测C-IAP2免疫荧光表达,用免疫印迹法检测C-IAP2表达变化。结果耐药株PANC-1/Gem组的IC_(50)值显著高于亲本株PANC-1组的IC_(50)值(P<0.05)。耐药株PANC-1/Gem组的倍增时间为32 h明显长于亲本株PANC-1组23 h(P<0.05)。2组细胞株在S期的比例差异无统计学意义(P>0.05)。与亲本株PANC-1组比较,耐药株PANC-1/Gem组表达出的C-IAP2荧光明显增强(P<0.05),C-IAP2蛋白表达水平显著升高(P<0.05)。结论间歇浓度递增法可诱导胰腺癌细胞株PANC-1对吉西他滨耐药,C-IAP2的上调表达在PANC-1/Gem耐药过程中起到一定作用。Objective To study the relationship between C-IAP2 expression and gemcitabine（ Gem） induced chemotherapy resistance of PANC-1pancreatic cancer cell line. Methods PANC-1 was obtained by gradient increase of concentration. Semi inhibitory concentration（ IC_（50））of the cells was detected by MTT assay. PANC-1 and PANC-1 / Gem cell doubling time was analyzed by cell counting assay. Cell cycle was analyzed by flow cytometry. C-IAP2 immunofluorescence expression was analyzed by immunofluorescence. C-IAP2 protein expression was detected by Western blot. Results IC_（50） value of PANC-1 / Gem was significantly higher than that of PANC-1（ P〈0. 05）. PANC-1 / Gem doubling time was significantly longer than that of PANC-1（ 32 h vs 23h）. There was No significant difference between PANC-1 / Gem and PANC-1 in S phase. Compared with PANC-1,PANC-1 / Gem expressed the C-IAP2 with a significantly enhanced immunofluorescence,the expression levels of C-IAP2 significantly increased（ P〈0. 05）.Conclusion Stable drug resistant of PANC-1 / Gem cell strain can be obtained by intermittent incremental concentrations of gemcitabine. The up-regulated expression of C-IAP2 may play a role to some extent in the process of drug resistance.

关 键 词：胰腺癌 细胞凋亡抑制蛋白2 多药耐药 吉西他滨 

分 类 号：R735.9[医药卫生—肿瘤]