高压氧治疗对血管性痴呆大鼠学习记忆能力的影响  

Hyperbaric oxygen therapy can improve the learning capacity and memory in vascular dementia

作　　者：曾喻[1] 潘福琼[1] 李雅娟[2] 彭梅[1] 黄玲岭[1] 陈福兰[1] 

机构地区：[1]四川省人民医院高压氧科,四川610072 [2]北京市通州区潞河医院高压氧科

出　　处：《中华物理医学与康复杂志》2016年第3期166-169,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：四川省卫生厅课题（080358）

摘　　要：目的探讨高压氧治疗（HBOT）对血管性痴呆（VD）大鼠学习记忆能力的影响。方法选择无特定病原体（SPF）级SD大鼠60只，雌雄不拘，按照随机数字表法将其分为正常组、假手术组、VD组、HBOT组，每组15只。采用改良四动脉方法建立VD模型，正常组不予特殊处理，假手术组手术中不灼烧椎动脉也不夹闭颈总动脉，HBOT组在VD组基础上进行HBOT7d。术后7d，分别对4组大鼠行Morris水迷宫实验检测其学习、记忆能力。结果与正常组比较，假手术组、VD组、HBOT组的平均逃避潜伏期较长（P〈0．05）。假手术组、VD组、HBOT组的平台象限穿越次数均少于正常组，平台象限游动距离均较短，其占总游程的百分比均低于正常组（P〈O．05）。与假手术组比较，VD组和HBOT组的平均逃避潜伏期较长（P〈0．05）。VD组、HBOT组的平台象限穿越次数均少于假手术组，平台象限游动距离均较短，其占总游程的百分比均低于假手术组（P〈0．05）。与VD组比较，HBOT组平均逃避潜伏期[（25．73±6．20）S]较短，平台象限穿越次数[（9．51±2．25）次]较多，平台象限游动距离[（494．72±208．26）次]较长，其占总游程的百分比[（28．71±5．32）％]较高，差异有统计学意义（P〈0．05）。结论HBOT可有效改善VD大鼠的学习记忆能力。Objective To explore the influence of hyperbaric oxygen therapy （HBOT） on the learning and memory ability using a rat model of vascular dementia （ VD）. Methods Sixty Sprague-Dawley rats were selected and divided into a normal group, a sham operation group, a VD group and an HBOT group, each of 15 rats using a random number table. The improved four-vessel method was used to establish a VD model in all except the rats in the normal group, but for the sham operation group, the vertebral artery was not subjected to firing and the arteria carotis communis was not clipped. The HBOT group was then given HBOT for 7 days. The Morris water maze test was used to evaluate their learning and memory ability of all 4 groups. Results Compared to the normal group, the mean escape latency of the sham operation group, the VD group and the HBOT group was significantly longer in each case （P〈0.05）. Their platform quadrant crossing times were also significantly less, and their quad- rant swimming distance as a proportion of their total swimming distance was significantly smaller （ P〈0.05 ）. Compared with the sham operation group, the mean escape latency of the VD and HBOT groups was significantly longer （P〈0.05） , the times of crossing platform quadrant were less and their quadrant swimming distance was a smaller proportion of their total swimming distance. As compared with the VD group, the mean escape latency of the HBOT group was significantly shorter [ （25.73±6.20） s] , the average platform quadrant crossing times were significantly more [ （9.51±2.25） times] and the platform quadrant swimming distance of the HBOT group was significantly longer [ （494.72±208.26）] , representing a significantly larger proportion of the total swimming distance [ （28.71 ±5.32） % ] （P〈0.05）. Conclusion HBOT can effectively improve learning and memory in vascular dementia, at least in rats.

关 键 词：高压氧治疗 血管性痴呆 MORRIS水迷宫 学习 记忆 

分 类 号：R749.13[医药卫生—神经病学与精神病学]