川黄合剂对TNF-α诱导的人腹膜间皮细胞IL-6和IL-8表达的影响  

Effect of Chuan-huang mixtures on TNF-α-induced IL-6 and IL-8 expressions in human peritoneal mesothelial cells in vitro

作　　者：朱桂松[1] 傅元冬[1] 陈畅泉[1] 

机构地区：[1]江苏省南京市中医院,江苏南京210001

出　　处：《现代中西医结合杂志》2016年第11期1169-1171,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：南京市医学科技发展项目(YKK14144)

摘　　要：目的探讨川黄合剂对TNF-α诱导的体外培养的人腹膜间皮细胞(HPMCs)增殖及白细胞介素-6(IL-6)和IL-8表达的影响。方法体外培养HPMCs,用含1%胎牛血清的RPMI-1640培养液同步24 h后,分为空白对照组、TNF-α诱导组(1μg/L)和低、中、高剂量川黄合剂干预组(n=3)。采用MTT法和流式细胞术检测各组细胞的增殖情况;采用酶联免疫吸附法(ELISA)检测细胞上清液中IL-6和IL-8蛋白含量,细胞沉淀用BCA蛋白检测方法测定细胞蛋白质含量,用以校正ELISA结果。结果干预48 h后,3种浓度川黄合剂干预组的OD值、PI值均显著高于TNF-α诱导组(P均<0.05),且IL-6和IL-8蛋白表达水平均明显低于TNF-α诱导组(P均<0.05)。结论川黄合剂能增强炎症状态下HPMCs活性,并降低炎症因子IL-6和IL-8的表达。Objective It is to investigate the effects of Chuan-huang mixtures on TNF- α-induced IL- 6 and IL- 8 expressions and proliferation in human peritoneal mesothelial cells（ HPMCs） in vitro. Methods Cell lines of HPMCs were subcultured and all experiments were performed using the cells at the third passage. After synchronization of cells growth with RPMI- 1640 culture solution containing 1% fetal bovine serum,HPMCs were divided into blank control group,TNF- α-induced（ 1 μg/L） group and TNF- α-induced plus low-,medium-and high-dose Chuan-huang mixtures groups. The proliferation of HPMCs was measured by MTT and flow cytometry assay. Proteins of IL- 6 and IL- 8 in culture supernatants were measured by enzyme linked immunosorbent assay（ ELISA）. Cell protein concentration was measured by trace bicinchoninic acid（ BCA）method to correct the ELISA assay results. Results After co- culturing by 48 h,the proliferation of HPMCs in TNF- α-induced plus low-,medium-and high-dose Chuan-huang mixtures groups were significantly increased compared to TNF- α-induced group（ all P〈0. 05）. Moreover,Chuan-huang mixtures could significantly decrease TNF- α-induced IL- 6 and IL-8 expressions in protein levels（ all P〈0. 05）. Conclusion Chuan-huang mixtures dose not only promote the cells proliferation,but also inhibit expressions of IL- 6 and IL- 8 in HPMCs cultured under inflammatory conditions.

关 键 词：川黄合剂 生大黄 川芎 白细胞介素-6 白细胞介素-8 急性腹膜炎 

分 类 号：R-33[医药卫生]