硒化合物在HepG2和Hela细胞中代谢生成硒蛋白的效应比较  

Production effect comparison of SEPP and GPx between HepG2 and Hela cells with different selenocompounds

作　　者：王琴[1] 高丽娜[2] 韩枫[1] 卢佳希[1] 刘轶群[1] 孙丽翠[1] 黄振武[1] 

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050 [2]北京武警总医院

出　　处：《卫生研究》2016年第2期259-262,共4页Journal of Hygiene Research

基　　金：国家自然科学基金面上项目(No.81372989);国家自然科学青年基金(No.31201352)

摘　　要：目的比较亚硒酸钠(Na_2SeO_3)、硒代蛋氨酸(SeMet)和甲基硒代半胱氨酸(MeSeCys)3种硒化合物在HepG2和Hela细胞中代谢生成硒蛋白P(SEPP)和谷胱甘肽过氧化物酶(GPx)的差异。方法将培养好的细胞分为对照组、Na_2SeO_3组、SeMet组和MeSeCys组。加入0.01μmol/L和0.1μmol/L的硒化合物作用48 h和72 h,收集细胞培养上清液和裂解液。采用酶联免疫吸附试验(ELISA)双抗体夹心法对上清中的SEPP和裂解液中的GPx进行定量检测。结果与对照组比较,0.1μmol/L SeMet和MeSeCys处理的Hela细胞生成的SEPP和GPx浓度显著升高(P<0.05)。与Hela细胞比较,0.1μmol/L 3种硒化合物处理的HepG2细胞生成的SEPP和GPx浓度均显著升高(P<0.05)。结论硒化合物在肝癌细胞HepG2中代谢生成硒蛋白的效应比在宫颈癌细胞Hela中更明显。Objective To compare the effect of several selenocompounds on the productions of SEPP and GPx in HepG2 and Hela cells.Methods The cultured HepG2 and Hela cells were divided into the control,Na_2SeO_3,SeMet and MeSeCys groups.After adding the selected selenocompounds（with the respective concentration 0.01 and 0.1μmol/L）,the experimental groups were then incubated for 48 h and 72 h.Finally,the cell culture supernatants and homogenates were collected for the SEPP and GPx concentrations detection by a double-antibody sandwich enzyme-linked immuno-sorbent assay（ELISA）.Results The SEPP and GPx concentrations in Hela cells treated with0.1 μmol/L SeMet and MeSeCys were significantly higher than that in the control group（P〈0.05）.The SEPP and GPx concentrations in HepG2 cell treated with 0.1 μmol/L selenocompounds were significantly higher than that in Hela cells（P〈0.05）.Conclusion HepG2 cells are more beneficial to the production of selenoproteins than Hela cells.

关 键 词：硒化合物 代谢 硒蛋白 HEPG2 胞 HELA细胞 

分 类 号：Q591[生物学—生物化学]