长链非编码RNA PCGEM1促进胰腺癌细胞增殖和迁移  

Long Non-coding RNA PCGEM1 Promotes the Proliferation and Migration of Human Pancreatic Cancer Cells

作　　者：徐岷[1] 倪鑫[1] 葛璐[1] 刘鑫[1] 周海浪[1] 黄红梅[1] 蒋小猛[1] 彭琬昕[2] 张尤历[1] 龚爱华[2] 

机构地区：[1]江苏大学附属医院消化内科,镇江212001 [2]江苏大学医学院基础医学系,镇江212013

出　　处：《中国细胞生物学学报》2016年第2期157-163,共7页Chinese Journal of Cell Biology

基　　金：江苏省卫生厅科研项目(批准号:H201434);江苏省"六大人才高峰"高层次人才选拔培养对象项目(批准号:2014-WSW-038);镇江市科技支撑计划项目(批准号:SH2014024)资助的课题~~

摘　　要：该文的目的为探讨长链非编码RNA PCGEM1(long non-coding RNA PCGEM1,lnc RNAPCGEM1)对胰腺癌细胞增殖和迁移的影响。通过荧光定量PCR检测三种胰腺癌细胞以及人胰腺癌组织和癌旁组织中PCGEM1的表达水平,分别在Bx PC3和Pa Tu8988细胞中转染p CDH-PCGEM1及其空载质粒p CDH,在SW1990细胞中转染si RNA和其对照si RNA,分别上调或下调PCGEM1在不同胰腺癌细胞中的表达;细胞克隆形成、CCK-8实验检测细胞增殖能力;Transwell实验检测细胞迁移能力;Western blot法检测MMP2、MMP9和E-钙黏着蛋白的蛋白表达以及荧光定量PCR检测MMP2和MMP9水平。结果表明,PCGEM1差异性表达于三种胰腺癌细胞中,其中SW1990表达水平相对较高,Bx PC3和Pa Tu8988表达水平相对较低。胰腺癌组织中PCGEM1水平高于癌旁组织。过表达PCGEM1后,细胞增殖和迁移能力增强,MMP2和MMP9的蛋白质水平增加,E-钙黏着蛋白的蛋白质水平降低;相反,降低PCGEM1表达,细胞增殖和迁移能力减弱,MMP2和MMP9的蛋白质水平降低,E-钙黏着蛋白的蛋白质水平升高。由此说明,lnc RNA PCGEM1可促进胰腺癌细胞的增殖和迁移。The aim of the present study was to investigate the effect of lnc RNA PCGEM1 on proliferation and migration in pancreatic cancer cells in human pancreatic cancer cells. The expression level of PCGEM1 in SW1990, Bx PC3 and Pa Tu8988 cell lines and in human pancreatic cancer tissues and their adjacent non-cancer tissues were detected by q RT-PCR. The p CDH-PCGEM1 was transfected into either Bx PC3 or Pa Tu8988 to increase the expression level of PCGEM1. Meanwhile, SW1990 transfected with si RNA to decrease the expression level of PCGEM1. Then, the abilities of the proliferation and migration were determined using clone formation assay,CCK-8 assay and cell migration assay, respectively. Furthermore, the protein levels of MMP2, MMP9 and Ecadherin were examined by Western blot and the expression levels of MMP2 and MMP9 were detected by q RTPCR. The results showed there were significant differences of PCGEM1 expression level in three kinds of human pancreatic cancer cell lines. The expression level of PCGEM1 in SW1990 was relatively high, compared with which was in Bx PC3 and Pa Tu8988. The level of PCGEM1 in pancreatic cancer tissues was higher than that in adjacent non-cancer tissues. Here, it was shown that knockdown of PCGEM1 suppressed proliferation in vitro. In contrast, the overexpression of PCGEM1 stimulated these processes. The expression level of PCGEM1 was positively correlated with the migration ability of human pancreatic cancer cells. In addition, after up regulation of PCGEM1, the expression of MMP2 and MMP9 increased while the expression of E-cadherin decreased. Conversely, the expression of MMP2 and MMP9 decreased and E-cadherin decreased after down regulation of PCGEM1. Taken together, lnc RNA PCGEM1 promotes the proliferation and migration of human pancreatic cancer cells.

关 键 词：长链非编码RNA PCGEM1 胰腺癌 增殖 迁移 

分 类 号：R735.9[医药卫生—肿瘤]