黄鳝大型多钩槽绦虫cox1基因的多态性研究  

Polymorphism of the Mitochodrial cox1 Gene of Polyonchobothrium magnum Isolated from Monopterus albus

作　　者：王芝英[1] 罗永莉[1] 

机构地区：[1]西南大学(荣昌校区)动物医学系,重庆荣昌402460

出　　处：《西南大学学报（自然科学版）》2016年第3期9-14,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(31172313)

摘　　要：为阐明黄鳝大型多钩槽绦虫渝西地区分离株线粒体基因细胞色素c氧化酶第I亚基（cox1）的遗传变异情况,本试验对黄鳝大型多钩槽绦虫cox1序列进行PCR扩增、克隆、测序及序列分析,并与GenBank中已知绦虫相应基因序列进行比较分析.结果显示渝西地区6个样品cox1碱基序列长度一致,均为444bp.同源分析发现渝西地区不同分离株间的同源性较高,为97.3%～100%,而6个大型多钩槽绦虫分离株与GenBank中收录的其他绦虫相比,同源性较低,为10.1%～12.1%.由于大型多钩槽绦虫cox1序列种内相对保守,种间差异较大,故可以作为种间遗传变异研究的标记.To investigate the genetic variation of the mitochondrial cytochrome c oxidase subunit 1（cox1）gene among Polyonchobothrium magnum（cox1）isolates which were collected from Monopterus albus in Western Chongqing area,cox1 genes from these strains were amplified using PCR amplification,cloned and sequenced,respectively.The homology analysis of these sequences were performed using BLAST and MegAlign programs of the software DNAStar version 5.0by comparing them with the corresponding sequences of nematodes available in GenBankTM.The results showed that all the six cox1 sequences of P.magnum samples were of the same length-444 bp.It was found P.magnumisolates from Monopterusalbus in Western Chongqing area had high homology among themselves（97.3%～100%）,while the similarity between P.magnum and the other tapeworms were low,ranging from 10.1% to12.1%.Since there is no significant variation in cox1 sequences within P.magnum,while inter-specific difference is obvious,it is concluded that cox1 sequences can be used as genetic markers for population genetic studies of P.magnum.The result of this study provides a scientific basis and theoretical reference on the molecular level and epidemiology survey of P.magnum.

关 键 词：黄鳝 大型多钩槽绦虫 cox1基因 PCR 序列分析 

分 类 号：S917.4[农业科学—水产科学]