地西他滨对恶性黑色素瘤细胞A375增殖、凋亡及侵袭的影响  

Effect of Decitabine on proliferation,apoptosis,invasion of malignant melanoma cell line A375

作　　者：霍文亮 弓军胜[1] 李晋福 王鹏[1] 张宝林[1] 

机构地区：[1]山西医科大学第一医院整形外科,山西太原030001 [2]山西医科大学研究生学院,山西太原030001

出　　处：《中国美容医学》2016年第3期39-43,共5页Chinese Journal of Aesthetic Medicine

基　　金：山西省卫生与计划生育委员会基金(2014032);山西医科大学第一医院院基金(YC1415)

摘　　要：目的:研究甲基化抑制剂地西他滨对人恶性黑色素瘤细胞A375增殖、凋亡及侵袭的影响。方法:采用不同浓度的地西他滨对A375细胞进行干预,用MTT实验检测地西他滨对细胞增殖的影响,用流式细胞仪检测细胞凋亡和细胞周期的变化,划痕愈合实验及Transwell小室实验观察细胞体外迁徙侵袭能力的改变。结果:与对照组相比,地西他滨组A375细胞的增殖活性受到明显抑制,凋亡比例显著增加,体外迁移和侵袭能力减弱,差异有统计学意义(P<0.05),地西他滨对A375细胞周期的影响则无显著差异(P>0.05)。结论:地西他滨能抑制恶性黑色素瘤A375细胞的增殖,其抗肿瘤效应有可能成为恶性黑色素瘤治疗的新途径。Objective To investigate the effect of Decitabine on proliferation,apoptosis, invasion of malignant melanoma cell line A375. Methods MTT assay was used to detect cell proliferation, flow cytometer was used to observe apoptosis and cell cycle, migration and movement capacity using scratch healing experiment, invasion using transwell assay. Results Decitabine inhibited the growth of A375 cells in a concentration- and time- dependent manner（P〈0.05）, After treatment with Decitabine for 24 h,the apoptosis rate was significantly higher in the treatment group over the control group（ P〈0.05）,the cell cycle of A375 cells was arrested at the G0/G1 phase（P〈0.05）, and the ability of migration and invasion were weakened（P〉0.05）. Conclusion Decitabine can inhibit proliferation of A375 cells,its anti-tumor activity may be a new way for the treatment of malignant melanoma.

关 键 词：黑色素瘤 地西他滨 增殖 凋亡 侵袭 

分 类 号：R739.5[医药卫生—肿瘤]