人巨细胞病毒基因在大肠癌组织的表达及其临床意义  

Clinical signifi cance of expression of human cytomegalovirus genes in colorectal cancer

作　　者：何云[1] 叶梦思[1] 周羽翙 林豪[1] 杨守醒 薛战雄[1] 薛向阳[1] 蔡振寨[1] 

机构地区：[1]温州医科大学附属第二医院消化内科,浙江省温州市325027

出　　处：《世界华人消化杂志》2016年第7期1024-1030,共7页World Chinese Journal of Digestology

基　　金：浙江省自然科学基金资助项目;No.LY15H160059;浙江省医药卫生科技基金资助项目;No.2016KYB192;温州市科技计划项目;No.Y20140691~~

摘　　要：目的:初步探讨人巨细胞病毒(human cytomegalovirus,HCMV)感染相关基因在大肠癌的表达及与大肠癌临床病理特征的关系.方法:巢式PCR方法检测60例大肠癌患者的肿瘤及癌旁正常肠黏膜组织HCMV UL135、UL136、US28及IE1基因;SDSPAGE凝胶电泳分析结合基因测序方法验证巢式PCR结果的准确性,用卡方检验或Fisher确切概率法比较两组间阳性率,Logistic回归分析HCMV感染与大肠癌患者临床病理特征的关系.结果:SDS-PAGE凝胶电泳分析结合基因测序方法验证了巢式PCR结果的准确性.UL135、UL136及US28基因在大肠癌组织表达的阳性率分别为35.0%、15.0%及60.0%,在癌旁正常肠组织的表达阳性率分别为16.7%、1.7%及18.3%,两组具有显著性差异(均P<0.05);IE1在大肠癌组织表达的阳性率(13.3%)与癌旁组织表达阳性率(10%)差异无显著意义.Logistic回归分析HCMV基因与大肠癌患者临床病理特征的关系,结果发现UL135、UL136及IE1基因表达与患者性别、年龄、肿瘤大小、病理分化程度、淋巴结转移及Dukes分期无关,而US28基因表达与淋巴结转移及Dukes分期相关,但和性别、年龄、肿块大小及病理分化程度无关.结论:大肠癌组织中UL135、UL136及US28基因表达较癌旁正常组织显著升高,其中US28阳性表达与大肠癌淋巴结转移及Dukes分期相关,提示HCMV某些基因表达可能参与大肠癌的发展.AIM：To detect the expression of human cytomegalovirus（HCMV） infection related genes in colorectal cancer tissues and their relationship with clinicopathological features of colorectal cancer.METHODS：HCMV UL135,UL136,US28 and IE1 gene expression in colorectal cancer tissues and corresponding adjacent normal samples was determined by nested PCR.The accuracy of nested PCR results was confirmed by SDSPAGE and DNA sequencing analysis.The relationship between HCMV gene expression and clinicopathological features of patients with colorectal cancer was also analyzed.Statistical methods included Chi-square test or Fisher exact probability test and logistic regression model analysis.RESULTS：The results of nested PCR were confirmed to be reliable.The positive expression rates of UL135,UL136 and US28 genes in the colorectal cancer tissues were 35.0%,15.0%and60.0%,respectively;and they were 16.7%,1.7%and 18.3%in corresponding normal tissues.The positive expression rates of UL135,UL136 and US28 genes in the colorectal cancer tissues were significantly higher than those in corresponding normal tissues（P 0.05 for all）.There was no significant difference in the positive expression rate of IE1 between colorectal cancer tissues（13.3%） and corresponding normal tissues（10%）.The expression of UL135,UL136 and IE1 genes had no significant association with gender,age,tumor size,histological differentiation,metastasis or Dukes stage.The expression of US28 had a significant association with lymph node metastasis and Dukes stage,but not with age,gender,tumor size or histo logical differentiation.CONCLUSION：UL135,UL136 and US28 gene expression is more often found in colorectal cancer tissues than in corresponding normal tissues,among which US28 has a significant association with lymph node metastasis and Dukes stage of colorectal cancer.Our findings suggest that some HCMV genes may play a role in the occurrence and development of colorectal cancer.

关 键 词：大肠癌 人巨细胞病毒 基因 巢式PCR 临床病理特征 

分 类 号：R735.34[医药卫生—肿瘤]