扁塑藤素对胃癌细胞MGC803及SGC7901增殖和凋亡的影响  

Pristimerin inhibits growth and induces apoptosis of gastric cancer MGC803 and SGC7901 cells

作　　者：赵钦府 袁学敏[2] 夏会[1] 吴琼[1] 姜开通[2] 

机构地区：[1]滨州医学院,山东省烟台市264000 [2]临沂市人民医院消化二科,山东省临沂市276000

出　　处：《世界华人消化杂志》2016年第7期1064-1069,共6页World Chinese Journal of Digestology

摘　　要：目的:研究扁塑藤素对胃癌MGC803和SGC7901细胞增殖及凋亡的影响,并初步探讨其可能的作用机制.方法:体外培养MGC803和SGC7901细胞,细胞接受0-50μmol/L药物处理之后,采用CCK-8实验、Annexin V-FITC/PI双染细胞凋亡检测及细胞周期检测实验研究扁塑藤素对胃癌MGC803细胞增殖及凋亡的影响;采用罗丹明123测定细胞线粒体跨膜电位变化,最后采用Western blot检测B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)和Bcl-2相关x蛋白(Bcl-associated x protein,Bax)表达水平.同时用人胃癌细胞系MGC803进行裸鼠成瘤实验,观察扁塑藤素对裸鼠成瘤的影响.结果:扁塑藤素可以抑制MGC803和SGC7901细胞增殖,其IC_(50)值分别为8.5μmol/L和12.6μmol/L;Annexin V-FITC/PI双染实验显示随着药物浓度的增加,出现凋亡的细胞明显增加;细胞周期实验显示扁塑藤素可以诱导MGC803细胞出现G_1期阻滞;细胞经过药物处理后,细胞线粒体跨膜电位明显下降.Western blot实验显示随着药物浓度的增加,细胞中Bax蛋白表达水平上升,Bcl-2蛋白表达水平下降,呈现一定的浓度依赖性.裸鼠成瘤实验显示,扁塑藤素可以抑制裸鼠种植瘤的生长.结论:扁塑藤素能显著抑制胃癌MGC803和SGC7901细胞增殖并诱导其凋亡,认为扁塑藤素可能通过增强线粒体通透性来诱导细胞凋亡.AIM：To investigate the effects of pristimerin on the growth and apoptosis of gastric cancer MGC803 and SGC7901 cells,and to preliminarily explore its action mechanism.METHODS：MGC803 and SGC7901 cells were cultured in vitro.After treatment with pristimerin,CCK-8 assay,Annexin V-FITC/PI staining,and cell cycle analysis were conducted to investigate the effects of pristimerin on the growth and apoptosis of MGC803 and SGC7901 cells,and then the mitochondrial membrane potential and Bax and Bcl-2 protein expression were determined by Rhodamine 123 staining and Western blot,respectively.Human gastric cancer MGC803 cells were transplanted to nude mice to establish an animal model,and the effect of pristimerin on tumor growth in this model was observed.RESULTS：Pristimerin inhibited MGC803 and SGC7901 cell growth,with the IC_（50） values of8.5 and 12.6 μmol/L,respectively.Annexin-V/PI staining showed that pristimerin induced the apoptosis of MGC803 and SGC7901 cells in a dose-dependent manner.The cell cycle analysis revealed that pristimerin could induce a G_1-phase arrest.Treatment with pristimerin significantly decreased the mitochondrial membrane potential.Treatment with pristimerin increased the expression of Bax protein and decreased the expression of Bd-2 in a concentration-dependent manner.Treatment with pristimerin could inhibit tumor growth in the nude mouse model.CONCLUSION：Pristimerin could inhibit proliferation and induce apoptosis of gastric cancer MGC803 and SGC7901 cells,possibly by inducing the loss of mitochondrial membrane potential.

关 键 词：扁塑藤素 胃癌细胞 Bcl-2相关x蛋白 B淋巴细胞瘤-2 

分 类 号：R735.2[医药卫生—肿瘤]