胰腺癌缺失基因DPC4在牙龈癌中的表达  

Expression of deleted in pancreatic cancer 4 in gingival cancer

作　　者：张强 刘继刚[2] 杨国海[3] 刘江 刘昊 

机构地区：[1]华北理工大学附属医院口腔科,唐山063000 [2]遵化市人民医院口腔科,遵化064200 [3]唐山工人医院分院口腔科,唐山063000 [4]华北理工大学基础医学院,唐山063000 [5]华北理工大学附属医院神经内科,唐山063000

出　　处：《安徽医科大学学报》2016年第4期598-600,共3页Acta Universitatis Medicinalis Anhui

基　　金：河北省2015年度医学科学研究重点课题计划项目(编号:20150087)

摘　　要：应用免疫组织化学技术和Real-timePCR法检测33例牙龈癌(高分化15例,中分化10例,低分化8例)和8例正常牙龈组织中DPC4蛋白和mRNA的表达情况。结果显示,牙龈癌组织DPC4阳性细胞百分比显著低于正常牙龈组织,高分化、中分化、低分化牙龈癌和牙龈组织的DPC4蛋白相对表达、mRNA表达差异有统计学意义(P<0.05)。提示牙龈癌DPC4表达降低,可能与牙龈癌的发生与发展有关。To observe the expression of DPC4 in gingival cancer tissue, the expression of protein and mRNA in 33 cases of gingival cancer and 8 cases of normal gum tissue were investigated by immunohistochemistry and Real-time PCR. Results showed that the positive cell rate of DPC4 in gingival cancer was lower than that in normal gum tis- sue. The expression of DPC4 and the expression of mRNA protein showed significant differences in well-differentia- ted, moderately differentiated, poorly differentiated gingival cancer and normal gum tissue （P 〈 0. 05）. The expres- sion of DPC4 in normal gum tissue is significantly higher than that in various differentiation types of gingival cancer tissue.

关 键 词：胰腺癌缺失基因4 牙龈癌 免疫组织化学 REAL-TIME PCR 

分 类 号：R780.2[医药卫生—口腔医学]