鱼腥草组分对生物膜的抑制作用及其应用  

Components of Houttuynia conrdata on the Inhibition of Bacterial Biofilm and Its Application

作　　者：李成[1,2] 娄在祥[1,2] 王洪新[1,2] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学国家功能食品工程技术研究中心,江苏无锡214122

出　　处：《食品与生物技术学报》2016年第3期284-289,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31201433)

摘　　要：研究了体积分数30%乙醇洗脱的鱼腥草柱层析组分对细菌生物膜的抑制活性,发现鱼腥草组分质量浓度在4.0 mg/m L的时候,对E.coli生物膜及Salmonella typhimurium生物膜形成的抑制率均为100%,同时鱼腥草组分在3.0 mg/m L和4.0 mg/m L时分别完全能够抑制E.coli和Salmonella typhimurium的生长。对鱼腥草组分进行成分分析,发现其主要成分为3,4-二羟基苯甲酸、绿原酸、芦丁、金丝桃苷、槲皮苷以及槲皮素。同时研究了鱼腥草柱层析组分对冷藏猪肉的保鲜效果,对微生物含量、挥发性盐基氮(TVB-N)、p H以及汁液流失率均有积极效果,在柱层析组分质量浓度为4.0 mg/m L的时候,每克肉品在15 d时微生物数量对数值达到5.95,而此时对照组微生物数量对数值为8.01。结果表明,鱼腥草柱层析组分在冷藏猪肉贮存中具有一定的应用潜力。The inhibition of bacterial biofilm activity by 30% ethanol elution of Houttuynia cordata was studied. The results showed that the inhibition rates of E.coli and Salmonella typhimurium biofilms were up to 100% when the extract concentration of H. cordata was 4.0 mg/m L. At the same time,the growth of E.coli and S.typhimurium were inhibited by H. cordata extract when its concentrations were 3.0 and 4.0 mg/m L,respectively. Further analysis of its extracts showed that3,4-dihydroxy benzoic acid,chlorogenic acid,rutin,hyperin,quercetin,and quercetin were its main components. Meanwhile,the effect of preservation of chilled pork by the extracts of H. cordata were also studied. The results exhibited that the microbial content,volatile base nitrogen（TVB-N）,p H and SAP turnover rate all had positive effects after the addition of the extractions. The total number of bacteria in per gram of the pork treated by 4.0 mg/m L extract was 5.95 at 15 th d,while the control group was 8.01. The results suggested that the extraction of H. cordata had potential applications in chilled pork during storage.

关 键 词：鱼腥草 生物膜 活性 冷藏肉 贮存 

分 类 号：TS251.44[轻工技术与工程—农产品加工及贮藏工程] TS202.3[轻工技术与工程—食品科学与工程]