Th2型细胞因子在胶原诱导性关节炎小鼠血清中的动态变化  

Dynamic changes of Th2 cytokines in serum of mice with collagen-induced arthritis

作　　者：赵晓菲[1] 安高[1] 封桂英[1] 刑恩鸿[2] 郭亚春[1] 宋鸿儒[1] 

机构地区：[1]承德医学院,河北承德067000 [2]承德医学院附属医院中心实验室,河北承德067000

出　　处：《职业与健康》2016年第4期471-474,共4页Occupation and Health

基　　金：国家自然科学基金面上项目(81273986)

摘　　要：目的研究鸡Ⅱ型胶原诱导性关节炎（CIA）小鼠血清中细胞因子白细胞介素（IL-4、IL-6和IL-10）的动态变化。方法采用完全随机化的分组方法将72只DBA1/J小鼠分为模型组和正常对照组,用制备的鸡Ⅱ型胶原乳剂免疫模型组小鼠,在加强免疫后的第7、14、21、35天无菌摘眼球取血,应用流式细胞术检测各组小鼠血清中细胞因子IL-4、IL-6、IL-10在不同免疫时间点的变化情况。结果模型组小鼠血清中IL-4水平在加强免疫后第7天显著升高（P〈0.05）,加强免疫后第14~35天有下降趋势,与正常对照组比较,差异无统计学意义（P〉0.05）;模型组小鼠血清IL-6水平在加强免疫后第7天和第14天上升明显（P〈0.05）,而第21、35天逐渐下降,但与正常对照组比较,差异无统计学意义（P〉0.05）。IL-10在CIA小鼠发病过程中,模型组与正常对照组比较,差异无统计学意义（P〉0.05）。结论 Th2型细胞因子IL-4和IL-6参与CIA小鼠发病过程,提示其与胶原诱导的关节炎的炎性反应进展有一定关系。[Objective] To study the dynamic changes of IL-4,IL-6 and IL-10 in the serum of mice with chicken type Ⅱcollageninduced arthritis（CIA）.[Methods] Using completely randomized grouping methods,72 DBA1/J mice were divided into the control group and the model group. The mice in model group were immunized by type Ⅱ collagen emulsion,and the blood samples were collected from eyeball under the sterile condition on the 7 th,14 th,21 st and 35 th day after booster immunization. The changes of serum IL-4,IL-6 and IL-10 at different time were detected by flow cytometry. [Results] The level of IL-4 in the model group significantly increased on the 7th day after booster immunization（P〈0.05）,it was on a downward trend between 14 th days and 35 th day,and there was no significant different between the control group and the model group（P〉0.05）. The level of IL-6 in the model group increased significantly on the 7th day and 14 th day after booster immunization（P〈0.05）,and it decreased gradually on the 21 st day and 35 th day,but the difference between the control group and the model group was not significant（P〉0.05）.There was no significant different in level of IL-10 between the model group and the control group in this experiment（P〉0.05）.[Conclusion] Th2 cytokines,including IL-4 and IL-6,participate in the pathogenesis of CIA in mice and their alterations at different time of the disease have a relation to the development of inflammatory reaction in CIA.

关 键 词：关节炎 胶原诱导 白介素 流式细胞术 

分 类 号：R-7332[医药卫生]