胰岛素样生长因子1受体对卵巢癌细胞增殖及化疗敏感性的影响  

Effect of insulin-like growth factor 1 receptor on cell proliferation and chemotherapy sensitivity of ovarian cancer

作　　者：刘恩令[1] 李君[1] 周玉秀[1] 王德华[2] 糜若然[2] 

机构地区：[1]河北医科大学附属唐山市工人医院,河北唐山063000 [2]天津医科大学总医院

出　　处：《山东医药》2016年第10期17-19,共3页Shandong Medical Journal

基　　金：河北省医学科学研究重点课题计划项目(ZD20140384)

摘　　要：目的观察胰岛素样生长因子1受体(IGF-1R)对卵巢癌细胞增殖及化疗敏感性的影响。方法将人卵巢癌HO8910PM细胞随机分为3组,A、B组分别转染IGF-1R siRNA及无义siRNA,C组不转染。采用实时PCR法检测IGF-1R mRNA,CCK-8法测定细胞增殖OD值,CCK-8法测定细胞对顺铂和卡铂半数有效抑制浓度(IC50)。结果转染48、72、96 h时,A组IGF-1R mRNA表达量均低于同时点B、C组(P均<0.05);转染72、96 h时,A组细胞增殖OD值低于同时点B、C组(P均<0.05);转染48 h时,A组细胞对顺铂和卡铂的IC50低于B、C组(P均<0.05);B、C组上述指标比较,P均>0.05。结论 IGF-1R可促进卵巢癌细胞增殖,降低卵巢癌细胞对顺铂、卡铂的化疗敏感性。Objective To observe the insulin-like growth factor 1 receptor（ IGF-1R） on cell proliferation and chemotherapy sensitivity of ovarian cancer. Methods Human ovarian cancer HO8910 PM cells were randomly divided into 3groups. Group A and B were respectively transfected by IGF-1R siRNA and nonsense siRNA,but group C without transfection. The real-time PCR was used to detect IGF-1R mRNA,CCK 8 was employed to detect OD value of cell proliferation,and CCK 8 was used to detect the effective inhibitory concentration（ IC50） on cisplatin and carboplatin. Results After transfection for 48,72 and 96 h,the expression of IGF-1R mRNA in the group A was lower than that of groups B and C（ all P 〈0. 05）; at 72 h and 96 h,the OD value of group A was lower than those of groups B and C（ all P 〈0. 05）; at 48 h,IC50value on cisplatin and carboplatin of group A was lower than those of groups B and C（ all P 〈0. 05）. No significant difference was found between groups B and C（ all P 〈0. 05）. Conclusion IGF-1R can promote the cell proliferation,and reduce the chemotherapy sensitivity of ovarian cancer cells to cisplatin and carboplatin.

关 键 词：卵巢癌 胰岛素样生长因子1受体 细胞增殖 顺铂 卡铂 化疗敏感性 

分 类 号：R737.31[医药卫生—肿瘤]