Study on intestinal transport of Veratrum alkaloids compatible with Panax ginseng across the Caco-2 cell monolayer model by UPLC-ESI-MS method  

作　　者：Ying-Hui Ma Meng-Ying Wei Yuan-Yuan Liu Feng-Rui Song Zhong-Ying Liu Zi-Feng Pi 

机构地区：[1]College of Pharmacy, Jilin University [2]Changchun Institute of Applied Chemistry, Chinese Academy of Sciences

出　　处：《Chinese Chemical Letters》2016年第2期215-220,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 81073040, 81274046, 81173507);the National Basic Research Program of China (National 973 Program) (Nos. 2011CB505300, 2011CB505305);Jilin Province Science and Technology Agency-funded projects (No.20150414040GH)

摘　　要：The Caco-2 cells have been recognized as effective tools to be applied to imitate the drug absorption in human intestine for the transport of drug. In this study, Caco-2 cell monolayer model was used to study compatibility of the transport of the Veratrum alkaloids in different proportions with Panax ginseng. A specific ultra-high performance liquid chromatographic-electrospray ionization-mass spectrometric（UPLC-ESI-MS） method is developed for the semi-quantitative determination of Veratrum alkaloids on intestinal transport with berberine as internal standard（IS）. In the Caco-2 model constructed, three influencing factors are investigated, including time, concentration and recovery rates of the Veratrum alkaloids during the uptake from AP（apical side） to BL（basolateral side）. The results suggest that the flux of Veratrum alkaloids is time dependent and concentration dependent. And the absorption of all eight Veratrum alkaloids increase after compatibility with Panax ginseng compared to the single Veratrum nigrum extraction. This research was studied from the perspective of intestinal absorption by the UPLCESI-MS method. This method was successfully applied to transport studies of the Veratrum alkaloids and the interaction mechanism between Veratrum nigrum and Panax ginseng.The Caco-2 cells have been recognized as effective tools to be applied to imitate the drug absorption in human intestine for the transport of drug. In this study, Caco-2 cell monolayer model was used to study compatibility of the transport of the Veratrum alkaloids in different proportions with Panax ginseng. A specific ultra-high performance liquid chromatographic-electrospray ionization-mass spectrometric（UPLC-ESI-MS） method is developed for the semi-quantitative determination of Veratrum alkaloids on intestinal transport with berberine as internal standard（IS）. In the Caco-2 model constructed, three influencing factors are investigated, including time, concentration and recovery rates of the Veratrum alkaloids during the uptake from AP（apical side） to BL（basolateral side）. The results suggest that the flux of Veratrum alkaloids is time dependent and concentration dependent. And the absorption of all eight Veratrum alkaloids increase after compatibility with Panax ginseng compared to the single Veratrum nigrum extraction. This research was studied from the perspective of intestinal absorption by the UPLCESI-MS method. This method was successfully applied to transport studies of the Veratrum alkaloids and the interaction mechanism between Veratrum nigrum and Panax ginseng.

关 键 词：UPLC-ESI-MS Veratrum nigrum ALKALOIDS Panax ginseng TRANSPORT Caco-2 cell monolayer model 

分 类 号：TQ464.4[化学工程—制药化工]