Notch1、VEGF在食管癌组织中的表达及与生存率的相关性  

Association between expression of Notch1 and VEGF and patients' survival rate in esophageal squamous cell carcinoma

作　　者：王丽萍 江小强 张海燕 焦丽梅[2] 

机构地区：[1]邯郸市峰峰矿区中医院,河北邯郸056200 [2]邯郸市第四医院,河北邯郸056200

出　　处：《中国热带医学》2016年第3期277-280,共4页China Tropical Medicine

摘　　要：目的探讨转染转移相关基因1(Notch1)和血管内皮生长因子(VEGF)在食管鳞癌组织中的表达情况及临床意义。方法收集食管鳞癌组织标本78例为实验组,并选取癌旁正常食管组织标本42份为对照组,采用EliVision^(TM)免疫组织化学法检测各组Notch1和VEGF的表达情况。Kaplari-Meier法计算患者的3年生存率,Notch1蛋白阳性表达和阴性表达患者的生存差别。结果食管鳞癌组织中Notch1和VEGF表达定位于细胞浆,表达水平与正常食管黏膜组织中的表达差异有统计学意义(P<0.05)。Notch1与肿瘤淋巴结转移、c TNM有关(P<0.05),VEGF的表达与肿瘤淋巴结转移、c TNM、分化程度有关(P<0.05);Notch1和VEGF之间表达呈负相关(P<0.05)。Notch1蛋白阳性表达患者的3年生存率显著低于Notch1蛋白阴性表达者(P<0.05)。结论 Notch1和VEGF在食管鳞癌的表达情况在其发生、发展和转移过程中起到一定的作用,并且其表达水平与预后直接相关,提示二者临床检测可为评估食管鳞癌严重程度提供参考。Objective To study the expression of Notch1 and VEGF in esophageal squamous cell carcinoma, and itsclinical significance. Methods Tissues of esophageal squamous cell carcinoma were obtained from 78 patients,and normaltissues distal from the tumors from 42 patients as controls. EliVision^TM was used to study expressions of Notch1 and VEGF, andKaplari-Meier method was applied to evaluate 3-year survival rate and its difference between patients with and without Notch1 expression in their tumors. Results Notch1 was expressed in the nucleus while VEGF expressed in the cytoplasm. The levelof expression of both genes was significantly different between tumor and normal tissues（P〈0.05 respectively）. Expression ofNotch1 was inversely correlated with that of VEGF（P〈0.05）, and both showed significant association with tumordifferentiation, lymph node metastasis and c TNM staging（P〈0.05 respectively）. Three- year survival rate of patients withNotch1-positive tumors was significantly lower than those with Notch1-negative tumors（P〈0.05）. Conclusion Expression of Notch1 and VEGF in esophageal squamous carcinoma may play an important role in carcinogenesis, tumor progression andmetastasis, and thus can be used to evaluate cancer pathogenesis and patients＇ prognosis.

关 键 词：食管鳞癌组织 转染转移相关基因1 血管内皮生长因子 EliVision^TM免疫组化 生存率 

分 类 号：R735.2[医药卫生—肿瘤]