不同培养方法对乳腺癌MDA-MB-231细胞DNA甲基化状态的影响  

Influence of different culture methods in DNA methylation status of breast cancer MDA-MB-231 cells

作　　者：乔莎[1] 黄映辉[2] 王世宝[1] 孙延霞[1] 卢振霞[1] 

机构地区：[1]吉林大学中日联谊医院肿瘤血液科,吉林长春130033 [2]北京工业大学生命科学与生物工程学院肿瘤研究所,北京100124

出　　处：《吉林大学学报（医学版）》2016年第2期271-276,I0004,共7页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(201215069)

摘　　要：目的:探讨不同细胞培养方法对乳腺癌MDA-MB-231细胞全基因组DNA甲基化状态的影响,阐明基因组甲基化状态与细胞生长环境的关系及其在肿瘤发生发展中的作用。方法:采用二维(2D)、三维(3D)细胞培养模型及小鼠肿瘤细胞原位移植(Ti)模型培养MDA-MB-231细胞并收集,分别用DNA提取试剂盒对收集的细胞进行DNA提取,DNA甲基化芯片检测3种模型培养后乳腺癌MDA-MB-231细胞全基因组DNA甲基化状态,应用Genomestudio软件计算每1个基因CpG位点的β值、DiffScore和Delta_Beta,并在2种模型之间比较,筛选出差异甲基化基因;采用DAVID在线分析工具对筛选的基因进行GO和Pathway分析。结果:3D与2D模型培养的MDA-MB-231细胞共发现480个差异甲基化基因(P<0.05),3D与Ti模型有86 448个差异甲基化基因(P<0.05),Ti与2D模型有90 005个差异甲基化基因(P<0.05);3D与2D、3D与Ti和Ti与2D模型的差异甲基化基因在多细胞生物体发育和细胞分化条目上有富集(P<0.05);亦在MAPK信号通路、细胞黏附分子和肌动蛋白骨架调节通路上有富集(P<0.05)。结论:采用2D、3D和Ti模型3种方法培养MDAMB-231细胞其全基因组DNA甲基化状态存在差异。Objective： To explore the influence of different cell culture methods in the genome-wide DNA methylation status of breast cancer MDA-MB-231 cells, and to clarify the relationship between genome-wide DNA methylation status and cell growth environment and the role of genome-wide DNA methylation status in the occurrence and development of tumor. Methods： The MDA-MB-231 cells were cultured with 2D and 3D cell culture models and mouse orthotopie transplantation model （Ti model） and collected, then DNA was extracted by DNA extraction kit and the genome-wide DNA methylation status of MDA-MB-231 cells after cultured with three different culture methods was detected by DNA methyiation chip, then the value of beta, DiffSeore and Delta_ Beta of the CpG loci of each gene were calculated by applying Genomestudio software, and the differential methylation genes were screened by Genomestudio software and GO and Pathway analysis of these genes were performed in DAVID on- line analysis tool. Flesults： All 480 genes of the MDA-MB-231 cells showed significant differences in the degree of methylation in 3D and 2D models （P〈0.05） ; 86 448 genes in 3D and Ti models （P〈0.05） ; 90 005 genes in Ti and 2D models （P〈0.05）. The differential methylation genes in 3D and 2D, 3D and Ti, and Ti and 2D models were enriched on the multicellular organismal development term and cell differentiation term （P〈0.05） ;also on MAPK signaling pathway, cell adhesion molecules （CAMs）, and regulation of actin cytoskeleton （P〈0.05）. Oonclusion.. There are differences in genome-wide DNA methylation status of MDA-MB-231 cells cultured in 2D, 3D cell culture and Ti models.

关 键 词：三维细胞培养 肿瘤 DNA甲基化 二维细胞培养 小鼠原位移植模型 

分 类 号：R73-35[医药卫生—肿瘤]