蜂蜜中泰乐菌素A的稳定性研究  

The stability of Tylosin A in honey

作　　者：陈惠兰[1] 张晓燕[1] 陈磊[1] 殷耀[1] 丁涛[1] 朱文君[1] 黄娟[1] 杨雯筌[1] 

机构地区：[1]江苏出入境检验检疫局动植物与食品检测中心国家蜂产品基准实验室,南京210001

出　　处：《中国蜂业》2016年第2期48-50,共3页Apiculture of China

摘　　要：泰乐菌素A对控制有土霉素抗性的美洲幼虫腐臭病很有效，且对蜜蜂的生长毒性较小，但过度使用会在蜂产品中残留。由于蜂蜜为弱酸性，泰乐菌素A在酸性条件下易降解。本文研究了不同温度下蜂蜜中泰乐菌素A的稳定性，并建立了蜂蜜中泰乐菌素A及其降解产物脱红霉糖泰乐菌素的测定方法：以pH6．0的磷酸盐缓冲液为提取剂，经HLB小柱净化富集后，采用高效液相色谱串联质谱法测定。该方法前处理简单，对泰乐菌素A和脱红霉糖泰乐菌素的检出限和定量限分别为1．0和2．0μg／kg，回收率范围为70．75～89．28％，R．SD为6．4～8．9％。方法回收率稳定且重现性较好，能满足日常检测工作的需要。Tylosin A is effective to AFB which is resistant to the antibiotic oxytetracycline and is almost non-toxic- ity to honeybees. However, tylosin A will remain in honey products if it was overdosed. Honey is weak acidic and ty- losin A will degrade in acidic conditions. Therefore, the degradation was studied in honey at different tempeture and the method for determination of tylosin A and the metabolite desmycosin was developed by high performance liquid chro- matography-tandem mass spectrometry. The antibiotics was extracted by phosphate buffer （pH6.0） and cleaned-up by HLB. The limit of detection and the limit of quantitation were 1.0μg/kg and 2.0μg/kg, respectively. The recoveries ranged from 70.75 % to 89.28 % while the precision from 6.4% to 8.9%. The method was used in detection.

关 键 词：蜂蜜 泰乐菌素A 脱红霉糖泰乐菌素 稳定性 高效液相色谱串联质谱 

分 类 号：S896.1[农业科学—特种经济动物饲养]