分心木中的化学成分及抗氧化活性研究  

Chemical Constituents from Diaphragma Juglandis Fructus and Their Antioxidant Activity

作　　者：赵焕新[1,2] 景援朝 白虹[2,3] 王英爱[2] 周洪雷[1] 

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东省医学科学院药物研究所,济南250062 [3]华中科技大学生命科学与技术学院,武汉430074

出　　处：《中国实验方剂学杂志》2016年第7期54-57,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072544)

摘　　要：目的：对中药分心木的化学成分进行分离鉴定,并对分得的单体化合物进行抗氧化活性考察。方法：应用各种柱色谱对分心木中的化合物进行分离、纯化,根据波谱数据鉴定其结构;采用1,1-二苯基-2-硝基苯肼（DPPH）自由基清除法对化合物进行抗氧化活性考察。结果：从分心木乙醇提取物中分离得到7个单体化合物,分别鉴定为二氢吐叶醇-9-O-β-D-葡萄糖苷（1）,紫杉叶素-3-O-α-L-呋喃阿拉伯糖苷（2）,槲皮素-3-O-（4″-O-乙酰基）-α-L-鼠李糖苷（3）,山柰酚-3-O-α-L-鼠李糖苷（4）,山柰酚（5）,柚皮素（6）和香草醛（7）。其中1-5,7为首次从分心木中分离得到。由于肾脏疾病的发病机制与机体的抗氧化平衡失调关系密切,为阐明分心木补肾功效的物质基础,结合前期的实验结果,采用DPPH自由基清除法考察了分心木中3个酚酸类及5个黄酮类化合物的抗氧化活性,发现8个化合物均显示不同程度的自由基清除能力。结论：分心木中的化学成分具有一定的抗氧化活性,为进一步阐明其传统功效提供了理论依据。Objective： To study on the chemical constituents of Diaphragma Juglandis Fructus and investigate the antioxidant activity of their monomeric compounds. Method： Various column chromatography methods were used to isolate and purify the compounds and their structures were identified on the basis of spectral data. In addition, the antioxidant activities of compounds were evaluated by 1, 1-diphenyl-2-picrylhydrazyl（ DPPH） free radical scavenging assay. Result： Seven monomeric compounds were isolated from ethanol extracts of Diaphragma Juglandis Fructus and their structures were identified as dihydrovomifoliol-9-O-β-D-glucopyranoside（ 1）, taxifolin-3-O-α-L-arabinofuranoside（ 2）, quercetin-3-O-（ 4″-O-acetyl）-α-L-rhamnopyranoside（ 3）,kaempferol-3-O-α-L-rhamnopyranoside（ 4）,kaempferol（ 5）,naringenin（ 6） and vanillin（ 7）. Compounds 1-5and 7 were isolated from Diaphragma Juglandis Fructus for the first time. As the pathogenesis of kidney disease is closely related to the body＇s disorder of antioxidant balance,in order to illustrate the material basis for kidneyreinforcing efficacy of Diaphragma Juglandis Fructus, three phenolic acid compounds and five flavonoids compounds were investigated for their antioxidant activity using DPPH radical scavenging assay combined with the previous experimental results. All the compounds indicated radical scavenging capacity to different extents.Conclusion： The chemical constituents of Diaphragma Juglandis Fructus have certain antioxidant activities,providing theoretical basis for the elaboration of its traditional efficacy.

关 键 词：分心木 化学成分 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]