菊苣根超微粉对免疫抑制小鼠肠道菌群的影响  

Effect of Superfine Powder of Cichorium intybus L.Root on Intestinal Microflora in Immunosuppressive Mice

作　　者：武炜[1] 

机构地区：[1]唐山师范学院生命科学系,唐山063000

出　　处：《天然产物研究与开发》2016年第3期429-432,共4页Natural Product Research and Development

基　　金：河北省高等学校科学技术研究指导项目(Z2010323);唐山市科学技术研究与发展项目(10120202C-1-3);唐山师范学院科学研究基金(2015B03)

摘　　要：本实验研究菊苣根超微粉对免疫抑制小鼠肠道菌群的影响,为菊苣的进一步开发利用提供理论依据。应用环磷酰胺(cyclophosphamide,CY)制备免疫功能抑制模型,小鼠分为空白对照组、CY模型组、菊苣根超微粉高剂量组、低剂量组。以菌落计数法检测其盲肠内容物中乳酸杆菌、双歧杆菌、肠杆菌和肠球菌的数量,以ELISA检测肠黏膜分泌型免疫球蛋白A(secretory immunoglobulin A,s Ig A)含量。与CY模型组相比,菊苣根超微粉高剂量组小鼠肠道的乳酸杆菌、双歧杆菌数量显著增加,大肠杆菌、肠球菌数量显著降低,肠道s Ig A含量显著升高。菊苣根超微粉对CY所致的免疫抑制小鼠肠道菌群紊乱有恢复作用。The aim of this study was to investigate the regulating effects of superfine powder of Cichorium intybus L. mot on intestinal microflora in immunosuppressive mice and to provide theoretical basis for further development and utiliza- tion of C. intybus. The mice model of intestinal dysbiosis was established by treating with cyclophosphamide. Mice were randomly divided into four groups： control group, eyclophosphamide （CY） model group, high concentration C. intybus root superfine powder group, low concentration C. intybus mot superfine powder group. The number of Lactobacillus, Bifidobacterium, Escherichia coli and Enterococcus in mice cecum contents was determined by colony counting and intesti- nal sIgA content was determined by ELISA assay. Compared with CY model group, the numbers of LactobaciUus and Bifidobacterium were significantly increased, the numbers of Escherlchla coli and Enterococcus were aignifieantly de- creased and the content of intestinal mucosal sIgA was significantly increased in high concentration C. intybus root super- fine powder group. These results indicated that C. intybus root superfine powder can regulate the disturbance of intestinal mieroflora in immunosuppresaive mice induced by CY.

关 键 词：菊苣 超微粉 环磷酰胺 免疫抑制小鼠 肠道菌群 分泌型免疫球蛋白A 

分 类 号：R285.5[医药卫生—中药学]