放射疗法对乳牙釉质硬度和微观形态的影响  

Effects of radiotherapy on micro-hardness and micro-morphology of deciduous tooth enamel

作　　者：陈满双 马文竹[1] 许小辉[1] 王燕[1] 林居红[1] 刘朝霞[1] 

机构地区：[1]重庆医科大学附属口腔医院儿童口腔科,口腔疾病与生物医学重庆市重点实验室,重庆市高校市级口腔生物医学工程重点实验室,重庆401147

出　　处：《上海交通大学学报（医学版）》2016年第3期344-348,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：2012年重庆市卫生局医学科研项目(2012-2-115);2013年重庆高校创新团队建设计划资助项目;重庆市高校市级口腔生物医学工程重点实验室资助项目~~

摘　　要：目的研究放射疗法对乳牙硬组织结构的影响。方法随机选取40个健康且新鲜拔除的滞留乳磨牙,沿近远中方向将牙齿切成颊舌两半,选择40个半切片用于实验。实验采用自身对照原则,照射之前采用显微硬度计对牙釉质进行初始硬度测试。之后分次完成40 Gy照射,2 Gy/次,1次/d,5 d/周,连续4周。每照射10 Gy后,采用显微硬度计对实验标本进行阶段硬度测试,所有照射剂量完成后采用显微硬度计观察乳牙牙釉质的显微硬度,扫描电子显微镜观察微观形态的变化。结果乳牙牙釉质的显微硬度随着照射剂量的增加而降低。扫描电子显微镜观察结果显示:照射过的牙釉质比未经过照射的牙釉质在所有位置上都表现出明显的微观表面形态改变。随着照射剂量的增加,表面形态发生渐进性破坏,表现为非晶体外观。结论随着照射剂量的增加,乳牙硬组织显微硬度降低,牙釉质的表面形态发生渐进破坏。Objective To investigate the effects of radiation therapy on hard tissue structure of deciduous teeth Methods Forty healthy and freshly extracted or exfoliated human deciduous molar teeth mesiodistally sectioned to yield buccal and lingual hemisections and forty vestibular sections were selected for enamel experiment following the principle of self-control.Enamel initial hardness was measured by micro-hardness tester prior to irradiation.Then a total of 40 Gy of irradiation was performed with 2 Gy/time,1 time/d,5 times/week for 4weeks.The enamel hardness was measured after each 10 Gy of irradiation.The micro-hardness of deciduous teeth was observed by micro-hardness tester after irradiation and micro morphological changes were observed by scanning electron microscopy（SEM）.Results The micro-hardness of deciduous teeth enamel decreased with radiation dose.SEM results showed that significant micro morphological changes in enamel surface were seen in all regions of irradiated enamel compared with non-irradiated enamel.With the increase in irradiation dose,surface morphology exhibited a progressive destruction,which appeared an amorphous appearance.Conclusion The micro-hardness of hard tissue of deciduous teeth decreases with radiation dose.Surface morphology of enamel exhibits a progressive destruction.

关 键 词：放射疗法 乳牙牙釉质 显微硬度 微观形态 

分 类 号：R739.91[医药卫生—肿瘤] R788[医药卫生—临床医学]